Nucleobindin 1

Nucleobindin-1 (NUCB1), also known as calnuc, is a protein that in humans is encoded by the NUCB1 gene.

NUCB1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1SNL
Identifiers
Aliases	NUCB1, CALNUC, NUC, nucleobindin 1
External IDs	OMIM: 601323; MGI: 97388; HomoloGene: 4507; GeneCards: NUCB1; OMA:NUCB1 - orthologs
Gene location (Human) Chr. Chromosome 19 (human) Band 19q13.33 Start 48,900,312 bp End 48,923,372 bp
Gene location (Mouse) Chr. Chromosome 7 (mouse) Band 7|7 B3 Start 45,139,882 bp End 45,159,830 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in stromal cell of endometrium Descending thoracic aorta right lobe of liver apex of heart ascending aorta left coronary artery right coronary artery right lobe of thyroid gland right auricle upper lobe of left lung Top expressed in external carotid artery choroid plexus of fourth ventricle internal carotid artery right kidney lip calvaria large intestine colon ileum duodenum More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding calcium ion binding metal ion binding protein binding G-protein alpha-subunit binding Cellular component cytoplasm lumenal side of Golgi membrane rough endoplasmic reticulum membrane cis-Golgi network Golgi-associated vesicle Golgi cisterna membrane extracellular region extrinsic component of Golgi membrane trans-Golgi network early endosome extracellular exosome nucleus endoplasmic reticulum-Golgi intermediate compartment extracellular space Golgi apparatus endoplasmic reticulum lumen Biological process regulation of protein targeting response to cisplatin post-translational protein modification Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 4924 18220 Ensembl ENSG00000104805 ENSMUSG00000030824 UniProt Q02818 Q02819 RefSeq (mRNA) NM_006184 NM_001163662 NM_008749 RefSeq (protein) NP_006175 NP_001157134 NP_032775 Location (UCSC) Chr 19: 48.9 – 48.92 Mb Chr 7: 45.14 – 45.16 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Structure

The human calnuc protein contains 461 amino acids and has a pI of 4.9.

The protein contains the following regions and domains:

• Signal peptide
• Leucine-zipper The leucine-zipper of calnuc may be responsible for its dimerization.
• aa 347-389 – Nuclear localization signal
• 2 zinc binding sites with high affinity (K_d = 20 nM)
• carboxypeptidase-like motifs : HFREXnH (aa 66) and HFTEXnH (aa 146)
• 2 EF-hands
• Potential anchor region– 15-aa hydrophobic region at its COOH terminus that may be sufficient for membrane anchoring

Further reading

• Miura K, Titani K, Kurosawa Y, Kanai Y (1992). "Molecular cloning of nucleobindin, a novel DNA-binding protein that contains both a signal peptide and a leucine zipper structure". Biochem. Biophys. Res. Commun. 187 (1): 375–80. doi:10.1016/S0006-291X(05)81503-7. PMID 1520323.
• Mochizuki N, Hibi M, Kanai Y, Insel PA (1995). "Interaction of the protein nucleobindin with G alpha i2, as revealed by the yeast two-hybrid system". FEBS Lett. 373 (2): 155–8. doi:10.1016/0014-5793(95)01031-9. PMID 7589456. S2CID 84629575.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Ballif BA, Mincek NV, Barratt JT, et al. (1996). "Interaction of cyclooxygenases with an apoptosis- and autoimmunity-associated protein". Proc. Natl. Acad. Sci. U.S.A. 93 (11): 5544–9. Bibcode:1996PNAS...93.5544B. doi:10.1073/pnas.93.11.5544. PMC 39283. PMID 8643612.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Kubota T, Miyauchi M, Miura K, et al. (1998). "Upregulation of nucleobindin expression in human-activated lymphocytes and non-Hodgkin's lymphoma". Pathol. Int. 48 (1): 22–8. doi:10.1111/j.1440-1827.1998.tb03823.x. PMID 9589460. S2CID 20338800.
• Lin P, Le-Niculescu H, Hofmeister R, et al. (1998). "The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein". J. Cell Biol. 141 (7): 1515–27. doi:10.1083/jcb.141.7.1515. PMC 2132997. PMID 9647645.
• Taniguchi N, Taniura H, Niinobe M, et al. (2000). "The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm". J. Biol. Chem. 275 (41): 31674–81. doi:10.1074/jbc.M005103200. PMID 10915798.
• Weiss TS, Chamberlain CE, Takeda T, et al. (2002). "Galpha i3 binding to calnuc on Golgi membranes in living cells monitored by fluorescence resonance energy transfer of green fluorescent protein fusion proteins". Proc. Natl. Acad. Sci. U.S.A. 98 (26): 14961–6. doi:10.1073/pnas.261572098. PMC 64966. PMID 11752444.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• de Alba E, Tjandra N (2004). "Structural studies on the Ca2+-binding domain of human nucleobindin (calnuc)". Biochemistry. 43 (31): 10039–49. doi:10.1021/bi049310a. PMID 15287731.
• Breuza L, Halbeisen R, Jenö P, et al. (2004). "Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46". J. Biol. Chem. 279 (45): 47242–53. doi:10.1074/jbc.M406644200. PMID 15308636.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
• Chen Y, Lin P, Qiu S, et al. (2007). "Autoantibodies to Ca2+ binding protein Calnuc is a potential marker in colon cancer detection" (PDF). Int. J. Oncol. 30 (5): 1137–44. doi:10.3892/ijo.30.5.1137. PMID 17390015.
• Tsukumo Y, Tomida A, Kitahara O, et al. (2007). "Nucleobindin 1 controls the unfolded protein response by inhibiting ATF6 activation". J. Biol. Chem. 282 (40): 29264–72. doi:10.1074/jbc.M705038200. PMID 17686766.