Nuestra Señora de la Asunción (Spanish for Our Lady of the Assumption) may refer to:
- Basilica of la Asunción de Nuestra Señora (Colmenar Viejo), a Gothic basilica located in Colmenar Viejo, Spain
- Cathedral of Córdoba, Argentina
- Our Lady of the Assumption Co-Cathedral, Baracoa, Cuba
- Church of Nuestra Señora de la Asunción (Meco), a church and parish located in Meco, Spain
- Church of Nuestra Señora de la Asunción (Valdemoro), a church located in Valdemoro, Spain
- Colegiata de Nuestra Señora de la Asunción, Catholic church built in the sixteenth century in the town of Osuna, in Andalusia, Spain
- Mission Nuestra Señora de la Asunción de Zía, a Spanish mission established 1706 in the area that is now New Mexico
- Nuestra Señora de la Asunción, Bujalance, a Roman Catholic church in Bujalance, Andalusia, southern Spain
- National Shrine and Cathedral-Parish of Our Lady of the Assumption of Maasin (Nuestra Señora de la Asunción de Maasin), locally known as the "Mahal nga Patrona sa Maasin", the Patroness of the City and Diocese in Maasin City, Southern Leyte, Philippines
- Santa Maria Church, the parish church of Santa Maria in Ilocos Sur province, Philippines
- Universidad Católica "Nuestra Señora de la Asunción", private pontifical university of Catholic obedience in Asunción, Paraguay
- Templo de Nuestra Señora de la Asunción, Jocotenango, church in Jocotenango, Guatemala