ORC3

Origin recognition complex subunit 3 is a protein that in humans is encoded by the ORC3 (ORC3L) gene.

Origin recognition complex (ORC) subunit 3 N-terminus
Identifiers
Symbol	ORC3_N
Pfam	PF07034
InterPro	IPR010748
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

ORC3
Identifiers
Aliases	ORC3, LAT, LATHEO, ORC3L, origin recognition complex subunit 3
External IDs	OMIM: 604972; MGI: 1354944; HomoloGene: 8225; GeneCards: ORC3; OMA:ORC3 - orthologs
Gene location (Human) Chr. Chromosome 6 (human) Band 6q15 Start 87,590,067 bp End 87,667,453 bp
Gene location (Mouse) Chr. Chromosome 4 (mouse) Band 4|4 A5 Start 34,570,796 bp End 34,614,944 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in triceps brachii muscle Achilles tendon ventricular zone glutes gonad embryo ganglionic eminence testicle right adrenal gland right adrenal cortex Top expressed in genital tubercle tail of embryo spermatocyte epiblast zygote ventricular zone maxillary prominence neural layer of retina mandibular prominence lens More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding DNA replication origin binding protein binding Cellular component nuclear origin of replication recognition complex origin recognition complex nucleus nucleoplasm nuclear pre-replicative complex DNA replication preinitiation complex nuclear body Biological process G1 phase DNA replication neural precursor cell proliferation DNA replication initiation pre-replicative complex assembly involved in nuclear cell cycle DNA replication G1/S transition of mitotic cell cycle Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 23595 50793 Ensembl ENSG00000135336 ENSMUSG00000040044 UniProt Q9UBD5 Q9JK30 RefSeq (mRNA) NM_001197259 NM_012381 NM_181837 NM_001159563 NM_015824 RefSeq (protein) NP_001184188 NP_036513 NP_862820 NP_001153035 NP_056639 Location (UCSC) Chr 6: 87.59 – 87.67 Mb Chr 4: 34.57 – 34.61 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Function

The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Studies of a similar gene in Drosophila suggested a possible role of this protein in neuronal proliferation and olfactory memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported for this gene.

Interactions

ORC3 has been shown to interact with:

• MCM4,
• MCM7,
• ORC2,
• ORC4,
• ORC5, and
• ORC6.

Further reading

• Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Springer J, Nanda I, Hoehn K, Schmid M, Grummt F (2000). "Identification and chromosomal localization of murine ORC3, a new member of the mouse origin recognition complex". Cytogenetics and Cell Genetics. 87 (3–4): 245–51. doi:10.1159/000015435. PMID 10702681. S2CID 29132152.
• Thome KC, Dhar SK, Quintana DG, Delmolino L, Shahsafaei A, Dutta A (Nov 2000). "Subsets of human origin recognition complex (ORC) subunits are expressed in non-proliferating cells and associate with non-ORC proteins". The Journal of Biological Chemistry. 275 (45): 35233–41. doi:10.1074/jbc.M005765200. PMID 10954718.
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A (Mar 2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Research. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
• Vashee S, Simancek P, Challberg MD, Kelly TJ (Jul 2001). "Assembly of the human origin recognition complex". The Journal of Biological Chemistry. 276 (28): 26666–73. doi:10.1074/jbc.M102493200. PMID 11323433.
• Dhar SK, Delmolino L, Dutta A (Aug 2001). "Architecture of the human origin recognition complex". The Journal of Biological Chemistry. 276 (31): 29067–71. doi:10.1074/jbc.M103078200. PMID 11395502.
• Kneissl M, Pütter V, Szalay AA, Grummt F (Mar 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". Journal of Molecular Biology. 327 (1): 111–28. doi:10.1016/S0022-2836(03)00079-2. PMID 12614612.
• Ramachandran N, Hainsworth E, Bhullar B, Eisenstein S, Rosen B, Lau AY, Walter JC, LaBaer J (Jul 2004). "Self-assembling protein microarrays". Science. 305 (5680): 86–90. Bibcode:2004Sci...305...86R. doi:10.1126/science.1097639. PMID 15232106. S2CID 20936301.
• Sibani S, Price GB, Zannis-Hadjopoulos M (May 2005). "Ku80 binds to human replication origins prior to the assembly of the ORC complex". Biochemistry. 44 (21): 7885–96. doi:10.1021/bi047327n. PMID 15910003.
• Matsuoka S, Ballif BA, Smogorzewska A, McDonald ER, Hurov KE, Luo J, Bakalarski CE, Zhao Z, Solimini N, Lerenthal Y, Shiloh Y, Gygi SP, Elledge SJ (May 2007). "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage". Science. 316 (5828): 1160–6. Bibcode:2007Sci...316.1160M. doi:10.1126/science.1140321. PMID 17525332. S2CID 16648052.