Octopine dehydrogenase family

In molecular biology, the octopine dehydrogenase family of enzymes act on the CH-NH substrate bond using NAD(+) or NADP(+) as an acceptor. The family includes octopine dehydrogenase EC 1.5.1.11, nopaline dehydrogenase EC 1.5.1.19, lysopine dehydrogenase EC 1.5.1.16 and opine dehydrogenase EC 1.5.1.-. NADPH is the preferred cofactor, but NADH is also used. Octopine dehydrogenase is involved in the reductive condensation of arginine and pyruvic acid to D-octopine.[1]

NAD/NADP octopine/nopaline dehydrogenase, alpha-helical domain
crystal structure of the n-(1-d-carboxylethyl)-l-norvaline dehydrogenase from arthrobacter sp. strain 1c
Identifiers
SymbolOctopine_DH
PfamPF02317
InterProIPR003421
SCOP21bg6 / SCOPe / SUPFAM
Available protein structures:
Pfam  structures / ECOD  
PDBRCSB PDB; PDBe; PDBj
PDBsumstructure summary

Opine dehydrogenases can be found in both bacteria and marine cephalopods. In bacteria, some of these opine dehydrogenases are involved in crown gall tumours that are produced by Agrobacterium spp., and which encode for the opine dehydrogenases on a Ti-plasmid. These bacteria can transfer a portion of this plasmid (T-DNA) to a susceptible plant cell; the T-DNA then integrates into the plant nuclear genome, where its genes can be expressed. Some of these genes direct the synthesis and secretion of unusual amino acid and sugar derivatives called opines - these opines are used as a carbon and sometimes a nitrogen source by the infecting bacteria.

Opine dehydrogenases are also found in the marine invertebrate cephalopods (octopuses, squid, and cuttlefish). For example, in marine cephalopods, octopine dehydrogenase activity in mantle muscle is significantly correlated with a species' ability to buffer the acidic end products of anaerobic metabolism, with activity declining strongly with a species' habitat depth.[2]

References

edit
  1. ^ Britton KL, Asano Y, Rice DW (July 1998). "Crystal structure and active site location of N-(1-D-carboxylethyl)-L-norvaline dehydrogenase". Nat. Struct. Biol. 5 (7): 593–601. doi:10.1038/854. PMID 9665174. S2CID 19976090.
  2. ^ Seibel BA, Thuesen EV, Childress JJ (April 2000). "Light-limitation on predator-prey interactions: consequences for metabolism and locomotion of deep-sea cephalopods". Biol. Bull. 198 (2): 284–98. doi:10.2307/1542531. JSTOR 1542531. PMID 10786948.
This article incorporates text from the public domain Pfam and InterPro: IPR003421