Our Lady of Guadalupe Church may refer to:
- in Mexico
- in the United States
- Our Lady of Guadalupe Catholic Church (Guadalupe, Arizona)
- Our Lady of Guadalupe Church (San Francisco, California)
- Our Lady of Guadalupe Church (Conejos, Colorado) (or Antonito, Colorado), listed on the National Register of Historic Places (NRHP) in Conejos County
- Our Lady of Guadalupe Church (Denver, Colorado), a Denver Landmark
- Our Lady of Guadalupe Chapel (New Orleans), Louisiana
- Our Lady of Guadalupe Parish (Taos, New Mexico)
- Our Lady of Guadalupe Church (New York City), New York
- Our Lady of Guadalupe Catholic Church (Houston), Texas
- Catedral Nuestra Señora de Guadalupe of Ponce, in Ponce, Puerto Rico, NRHP-listed