Polymerase delta-interacting protein 2 also known as Polymerase delta-interacting protein of 38 kDa (PDIP38) is encoded by the POLDIP2 gene in humans.[ 5] [ 6]
This gene encodes a protein that interacts with the DNA polymerase delta p50 subunit. The encoded protein also interacts with proliferating cell nuclear antigen. Some transcripts of this gene overlap in a tail-to-tail orientation with the gene for tumor necrosis factor, alpha-induced protein 1 (TNFAIP1).[ 6] POLDIP2 / PDIP38 is a moonlighting protein that has been identified in both the nucleus [ 7] and the mitochondrion [ 8] [ 9]
In the nucleus, POLDIP2 has been shown to interact with PCNA ,[ 5] while in the mitochondrion POLDIP2 has been shown to interact with proteins constituting the mitochondrial DNA nucleoid [ 10] and the AAA + protease CLPXP [ 9] [ 11] where it modulates both the specificity and stability of this proteolytic machine.
^ a b c GRCh38: Ensembl release 89: ENSG00000004142 – Ensembl , May 2017
^ a b c GRCm38: Ensembl release 89: ENSMUSG00000001100 – Ensembl , May 2017
^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .
^ a b Liu L, Rodriguez-Belmonte EM, Mazloum N, Xie B, Lee MY (March 2003). "Identification of a novel protein, PDIP38, that interacts with the p50 subunit of DNA polymerase delta and proliferating cell nuclear antigen" . The Journal of Biological Chemistry . 278 (12): 10041–7. doi :10.1074/jbc.M208694200 . PMID 12522211 .
^ a b "Entrez Gene: POLDIP2 polymerase (DNA-directed), delta interacting protein 2" .
^ Wong A, Zhang S, Mordue D, Wu JM, Zhang Z, Darzynkiewicz Z, et al. (October 2013). "PDIP38 is translocated to the spliceosomes/nuclear speckles in response to UV-induced DNA damage and is required for UV-induced alternative splicing of MDM2" . Cell Cycle . 12 (19): 3184–93. doi :10.4161/cc.26221 . PMC 3865014 . PMID 23989611 .
^ Xie B, Li H, Wang Q, Xie S, Rahmeh A, Dai W, Lee MY (June 2005). "Further characterization of human DNA polymerase delta interacting protein 38" . The Journal of Biological Chemistry . 280 (23): 22375–84. doi :10.1074/jbc.M414597200 . PMID 15811854 .
^ a b Strack PR, Brodie EJ, Zhan H, Schuenemann VJ, Valente LJ, Saiyed T, et al. (November 2020). "Polymerase delta-interacting protein 38 (PDIP38) modulates the stability and activity of the mitochondrial AAA+ protease CLPXP" . Communications Biology . 3 (1): 646. doi :10.1038/s42003-020-01358-6 . PMC 7647994 . PMID 33159171 .
^ Cheng X, Kanki T, Fukuoh A, Ohgaki K, Takeya R, Aoki Y, et al. (December 2005). "PDIP38 associates with proteins constituting the mitochondrial DNA nucleoid". Journal of Biochemistry . 138 (6): 673–8. doi :10.1093/jb/mvi169 . PMID 16428295 .
^ Paredes F, Sheldon K, Lassègue B, Williams HC, Faidley EA, Benavides GA, et al. (February 2018). "Poldip2 is an oxygen-sensitive protein that controls PDH and αKGDH lipoylation and activation to support metabolic adaptation in hypoxia and cancer" . Proceedings of the National Academy of Sciences of the United States of America . 115 (8): 1789–1794. Bibcode :2018PNAS..115.1789P . doi :10.1073/pnas.1720693115 . PMC 5828627 . PMID 29434038 .
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Hartley JL, Temple GF, Brasch MA (November 2000). "DNA cloning using in vitro site-specific recombination" . Genome Research . 10 (11): 1788–95. doi :10.1101/gr.143000 . PMC 310948 . PMID 11076863 .
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Xie B, Li H, Wang Q, Xie S, Rahmeh A, Dai W, Lee MY (June 2005). "Further characterization of human DNA polymerase delta interacting protein 38" . The Journal of Biological Chemistry . 280 (23): 22375–84. doi :10.1074/jbc.M414597200 . PMID 15811854 .
Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, et al. (September 2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell . 122 (6): 957–68. doi :10.1016/j.cell.2005.08.029 . hdl :11858/00-001M-0000-0010-8592-0 . PMID 16169070 . S2CID 8235923 .
Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, et al. (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature . 437 (7062): 1173–8. Bibcode :2005Natur.437.1173R . doi :10.1038/nature04209 . PMID 16189514 . S2CID 4427026 .
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Klaile E, Müller MM, Kannicht C, Otto W, Singer BB, Reutter W, et al. (September 2007). "The cell adhesion receptor carcinoembryonic antigen-related cell adhesion molecule 1 regulates nucleocytoplasmic trafficking of DNA polymerase delta-interacting protein 38" . The Journal of Biological Chemistry . 282 (36): 26629–40. doi :10.1074/jbc.M701807200 . PMID 17623671 .