Vacutainer was developed in 1947 by Joseph Kleiner. The vein is first punctured with the hypodermic needle, which is connected to a translucent plastic holder. The needle actually has a second, smaller needle, and when a Vacutainer test tube is pushed down into the holder, its rubber cap is pierced. The vacuum in the tube sucks blood though the needle and fills itself. The tube is then removed and another can be inserted and filled the same way. It is important to remove the tube before withdrawing the needle, as there may still be some suction left, causing pain upon withdrawal. (More...)