Prostaglandin D2 synthase

Prostaglandin-H2 D-isomerase (PTGDS) is an enzyme that in humans is encoded by the PTGDS gene.

PTGDS
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4OS8, 2WWP, 3O19, 3O22, 3O2Y, 4IMN, 4IMO, 4ORR, 4ORS, 4ORU, 4ORW, 4ORX, 4ORY, 4OS0, 4OS3
Identifiers
Aliases	PTGDS, L-PGDS, LPGDS, PDS, PGD2, PGDS, PGDS2, prostaglandin D2 synthase
External IDs	OMIM: 176803; MGI: 99261; HomoloGene: 737; GeneCards: PTGDS; OMA:PTGDS - orthologs
Gene location (Human) Chr. Chromosome 9 (human) Band 9q34.3 Start 136,975,092 bp End 136,981,742 bp
Gene location (Mouse) Chr. Chromosome 2 (mouse) Band 2 A3|2 17.28 cM Start 25,356,721 bp End 25,360,058 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in left testis right testis C1 segment substantia nigra putamen apex of heart right auricle temporal lobe amygdala caudate nucleus Top expressed in vestibular membrane of cochlear duct olfactory tubercle median eminence arcuate nucleus mammillary body iris optic nerve pontine nuclei retinal pigment epithelium stria vascularis More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transporter activity isomerase activity fatty acid binding prostaglandin-D synthase activity protein binding small molecule binding retinoid binding Cellular component cytoplasm Golgi apparatus rough endoplasmic reticulum nuclear membrane endoplasmic reticulum membrane membrane extracellular region endoplasmic reticulum perinuclear region of cytoplasm extracellular exosome nucleus extracellular space Biological process prostaglandin metabolic process lipid metabolism cyclooxygenase pathway fatty acid metabolic process fatty acid biosynthetic process prostaglandin biosynthetic process regulation of circadian sleep/wake cycle, sleep transport Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5730 19215 Ensembl ENSG00000107317 ENSMUSG00000015090 UniProt P41222 Q5SQ11 O09114 RefSeq (mRNA) NM_000954 NM_008963 RefSeq (protein) NP_000945 NP_032989 Location (UCSC) Chr 9: 136.98 – 136.98 Mb Chr 2: 25.36 – 25.36 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Function

The protein encoded by this gene is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of prostaglandin H₂ (PGH₂) to prostaglandin D₂ (PGD₂). PGD₂ functions as a neuromodulator as well as a trophic factor in the central nervous system. PGD₂ is also involved in smooth muscle contraction/relaxation and is a potent inhibitor of platelet aggregation. This gene is preferentially expressed in brain. Studies with transgenic mice over-expressing this gene suggest that this gene may be also involved in the regulation of non-rapid eye movement sleep. Furthermore, PTGDS and its product PGD₂ are elevated in the bald-scalp areas of men with male pattern baldness (androgenetic alopecia).

Clinical use

Prostaglandin D₂ synthase is used clinically as a diagnostic marker for liquorrhea, that is, to check whether fluid leaking from the nose or ear contains cerebrospinal fluid. This is important in the assessment of head trauma severity. In a medical context, the older term "beta-trace protein" is frequently used to refer to PTGDS.

See also

• Hematopoietic prostaglandin D synthase

Further reading

• White DM, Mikol DD, Espinosa R, Weimer B, Le Beau MM, Stefansson K (Nov 1992). "Structure and chromosomal localization of the human gene for a brain form of prostaglandin D2 synthase". The Journal of Biological Chemistry. 267 (32): 23202–8. doi:10.1016/S0021-9258(18)50077-6. PMID 1385416.
• Kuruvilla AP, Hochwald GM, Ghiso J, Castaño EM, Pizzolato M, Frangione B (Nov 1991). "Isolation and amino terminal sequence of beta-trace, a novel protein from human cerebrospinal fluid". Brain Research. 565 (2): 337–40. doi:10.1016/0006-8993(91)91666-O. PMID 1726844. S2CID 54405256.
• Zahn M, Mäder M, Schmidt B, Bollensen E, Felgenhauer K (May 1993). "Purification and N-terminal sequence of beta-trace, a protein abundant in human cerebrospinal fluid". Neuroscience Letters. 154 (1–2): 93–5. doi:10.1016/0304-3940(93)90179-O. PMID 7689714. S2CID 42018337.
• Harrington MG, Aebersold R, Martin BM, Merril CR, Hood L (1993). "Identification of a brain-specific human cerebrospinal fluid glycoprotein, beta-trace protein". Applied and Theoretical Electrophoresis. 3 (5): 229–34. PMID 7692978.
• Bastien L, Sawyer N, Grygorczyk R, Metters KM, Adam M (Apr 1994). "Cloning, functional expression, and characterization of the human prostaglandin E2 receptor EP2 subtype". The Journal of Biological Chemistry. 269 (16): 11873–7. doi:10.1016/S0021-9258(17)32654-6. PMID 8163486.
• Funk CD, Furci L, FitzGerald GA, Grygorczyk R, Rochette C, Bayne MA, Abramovitz M, Adam M, Metters KM (Dec 1993). "Cloning and expression of a cDNA for the human prostaglandin E receptor EP1 subtype". The Journal of Biological Chemistry. 268 (35): 26767–72. doi:10.1016/S0021-9258(19)74379-8. PMID 8253813.
• Abramovitz M, Boie Y, Nguyen T, Rushmore TH, Bayne MA, Metters KM, Slipetz DM, Grygorczyk R (Jan 1994). "Cloning and expression of a cDNA for the human prostanoid FP receptor". The Journal of Biological Chemistry. 269 (4): 2632–6. doi:10.1016/S0021-9258(17)41991-0. PMID 8300593.
• Hoffmann A, Conradt HS, Gross G, Nimtz M, Lottspeich F, Wurster U (Aug 1993). "Purification and chemical characterization of beta-trace protein from human cerebrospinal fluid: its identification as prostaglandin D synthase". Journal of Neurochemistry. 61 (2): 451–6. doi:10.1111/j.1471-4159.1993.tb02145.x. PMID 8336134. S2CID 34300607.
• Leone MG, Saso L, Del Vecchio A, Mo MY, Silvestrini B, Cheng CY (Aug 1993). "Micropurification of two human cerebrospinal fluid proteins by high performance electrophoresis chromatography". Journal of Neurochemistry. 61 (2): 533–40. doi:10.1111/j.1471-4159.1993.tb02156.x. PMID 8336140. S2CID 29843974.
• Giacomelli S, Leone MG, Grima J, Silvestrini B, Cheng CY (Feb 1996). "Astrocytes synthesize and secrete prostaglandin D synthetase in vitro". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1310 (3): 269–76. doi:10.1016/0167-4889(95)00182-4. PMID 8599604.
• Blödorn B, Mäder M, Urade Y, Hayaishi O, Felgenhauer K, Brück W (May 1996). "Choroid plexus: the major site of mRNA expression for the beta-trace protein (prostaglandin D synthase) in human brain". Neuroscience Letters. 209 (2): 117–20. doi:10.1016/0304-3940(96)12614-8. PMID 8761996. S2CID 34159760.
• Bonaldo MF, Lennon G, Soares MB (Sep 1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Yamashima T, Sakuda K, Tohma Y, Yamashita J, Oda H, Irikura D, Eguchi N, Beuckmann CT, Kanaoka Y, Urade Y, Hayaishi O (Apr 1997). "Prostaglandin D synthase (beta-trace) in human arachnoid and meningioma cells: roles as a cell marker or in cerebrospinal fluid absorption, tumorigenesis, and calcification process". The Journal of Neuroscience. 17 (7): 2376–82. doi:10.1523/JNEUROSCI.17-07-02376.1997. PMC 6573504. PMID 9065498.
• White DM, Takeda T, DeGroot LJ, Stefansson K, Arnason BG (May 1997). "Beta-trace gene expression is regulated by a core promoter and a distal thyroid hormone response element". The Journal of Biological Chemistry. 272 (22): 14387–93. doi:10.1074/jbc.272.22.14387. PMID 9162076.
• Eguchi Y, Eguchi N, Oda H, Seiki K, Kijima Y, Matsu-ura Y, Urade Y, Hayaishi O (Dec 1997). "Expression of lipocalin-type prostaglandin D synthase (beta-trace) in human heart and its accumulation in the coronary circulation of angina patients". Proceedings of the National Academy of Sciences of the United States of America. 94 (26): 14689–94. Bibcode:1997PNAS...9414689E. doi:10.1073/pnas.94.26.14689. PMC 25094. PMID 9405674.
• Tokugawa Y, Kunishige I, Kubota Y, Shimoya K, Nobunaga T, Kimura T, Saji F, Murata Y, Eguchi N, Oda H, Urade Y, Hayaishi O (Feb 1998). "Lipocalin-type prostaglandin D synthase in human male reproductive organs and seminal plasma". Biology of Reproduction. 58 (2): 600–7. doi:10.1095/biolreprod58.2.600. PMID 9475419.
• Saso L, Leone MG, Sorrentino C, Giacomelli S, Silvestrini B, Grima J, Li JC, Samy E, Mruk D, Cheng CY (Nov 1998). "Quantification of prostaglandin D synthetase in cerebrospinal fluid: a potential marker for brain tumor". Biochemistry and Molecular Biology International. 46 (4): 643–56. doi:10.1080/15216549800204172. PMID 9844724. S2CID 29770550.
• Blödorn B, Brück W, Tumani H, Michel U, Rieckmann P, Althans N, Mäder M (Sep 1999). "Expression of the beta-trace protein in human pachymeninx as revealed by in situ hybridization and immunocytochemistry". Journal of Neuroscience Research. 57 (5): 730–4. doi:10.1002/(SICI)1097-4547(19990901)57:5<730::AID-JNR14>3.0.CO;2-7. PMID 10462696. S2CID 23555031.
• Pinzar E, Kanaoka Y, Inui T, Eguchi N, Urade Y, Hayaishi O (Apr 2000). "Prostaglandin D synthase gene is involved in the regulation of non-rapid eye movement sleep". Proceedings of the National Academy of Sciences of the United States of America. 97 (9): 4903–7. Bibcode:2000PNAS...97.4903P. doi:10.1073/pnas.090093997. PMC 18330. PMID 10781097.

External links

• Overview of all the structural information available in the PDB for UniProt: P41222 (Human Prostaglandin-H2 D-isomerase) at the PDBe-KB.
• Overview of all the structural information available in the PDB for UniProt: O09114 (Mouse Prostaglandin-H2 D-isomerase) at the PDBe-KB.