Protein N-terminal methyltransferase (EC 2.1.1.244, NMT1 (gene), METTL11A (gene)) is an enzyme with systematic name S-adenosyl-L-methionine:N-terminal-(A,P,S)PK-(protein) methyltransferase.[1][2] This enzyme catalyses the following chemical reaction
| Protein N-terminal methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.1.1.244 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- (1) 3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein] (overall reaction)
- (1a) S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-(A,S)PK-[protein]
- (1b) S-adenosyl-L-methionine + N-terminal-N-methyl-N-(A,S)PK-[protein] S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-(A,S)PK-[protein]
- (1c) S-adenosyl-L-methionine + N-terminal-N,N-dimethyl-N-(A,S)PK-serine-[protein] S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein]
- (2) 2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein] (overall reaction)
- (2a) S-adenosyl-L-methionine + N-terminal-PPK-[protein] S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-PPK-[protein]
- (2b) S-adenosyl-L-methionine + N-terminal-N-methyl-N-PPK-[protein] S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein]
This enzyme methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys.
References
edit- ^ Webb KJ, Lipson RS, Al-Hadid Q, Whitelegge JP, Clarke SG (June 2010). "Identification of protein N-terminal methyltransferases in yeast and humans". Biochemistry. 49 (25): 5225–35. doi:10.1021/bi100428x. PMC 2890028. PMID 20481588.
- ^ Tooley CE, Petkowski JJ, Muratore-Schroeder TL, Balsbaugh JL, Shabanowitz J, Sabat M, Minor W, Hunt DF, Macara IG (August 2010). "NRMT is an alpha-N-methyltransferase that methylates RCC1 and retinoblastoma protein". Nature. 466 (7310): 1125–8. doi:10.1038/nature09343. PMC 2939154. PMID 20668449.
External links
edit- Protein+N-terminal+methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
