Ptáček (feminine Ptáčková) is a Czech surname (meaning "little bird"), it may refer to:
- Adam Ptáček (born 1980), Czech pole vaulter
- Dana Ptáčková, Czech basketball player
- Fátima Ptacek (born 2000), American child actor and model
- František Ptáček, Czech professional ice hockey player
- Hynek Ptáček of Pirkstein (1404–1444), Czech nobleman
- Joe Ptacek, vocalist
- Kathryn Ptacek (born 1952, Omaha, Nebraska), American author and editor
- Rainer Ptacek (1951–1997), American Tucson-based guitarist and singer-songwriter
- Robert "Bob" Ptacek, professional American and Canadian football quarterback
- Vladimír Ptáček, Czech basketball player