Pyrimidine oxygenase (EC 1.14.99.46, RutA) is an enzyme with systematic name uracil,FMNH2:oxygen oxidoreductase (uracil hydroxylating, ring-opening).[1][2] This enzyme catalyses the following chemical reaction
| Pyrimidine oxygenase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.99.46 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- (1) uracil + FMNH2 + O2 (Z)-3-ureidoacrylate peracid + FMN
- (2) thymine + FMNH2 + O2 (Z)-2-methylureidoacrylate peracid + FMN
In vitro the product (Z)-3-ureidoacrylate peracid is spontaneously reduced to ureidoacrylate.
References
edit- ^ Mukherjee T, Zhang Y, Abdelwahed S, Ealick SE, Begley TP (April 2010). "Catalysis of a flavoenzyme-mediated amide hydrolysis". Journal of the American Chemical Society. 132 (16): 5550–1. doi:10.1021/ja9107676. PMC 2873085. PMID 20369853.
- ^ Kim KS, Pelton JG, Inwood WB, Andersen U, Kustu S, Wemmer DE (August 2010). "The Rut pathway for pyrimidine degradation: novel chemistry and toxicity problems". Journal of Bacteriology. 192 (16): 4089–102. doi:10.1128/JB.00201-10. PMC 2916427. PMID 20400551.
External links
edit- Pyrimidine+oxygenase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
