RMND5B

Required for meiotic nuclear division 5 homolog B (S. cerevisiae), also known as RMND5B, is a protein which in humans is encoded by the RMND5B gene. It has a zinc finger domain and is highly conserved throughout many eukaryotic organisms.

RMND5B
Identifiers
Aliases	RMND5B, GID2, GID2B, required for meiotic nuclear division 5 homolog B
External IDs	MGI: 1913339; HomoloGene: 100777; GeneCards: RMND5B; OMA:RMND5B - orthologs
Gene location (Human) Chr. Chromosome 5 (human) Band 5q35.3 Start 178,130,996 bp End 178,150,568 bp
Gene location (Mouse) Chr. Chromosome 11 (mouse) Band 11|11 B1.3 Start 51,514,498 bp End 51,526,723 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in left testis right testis apex of heart gastrocnemius muscle muscle of thigh prefrontal cortex epithelium of esophagus skin of leg anterior pituitary ectocervix Top expressed in granulocyte interventricular septum spermatid hair follicle lip spermatocyte morula dentate gyrus of hippocampal formation granule cell seminiferous tubule right kidney More reference expression data BioGPS More reference expression data
Gene ontology Molecular function ubiquitin-protein transferase activity protein binding transferase activity metal ion binding Cellular component nucleus cytoplasm GID complex cytosol Biological process proteasome-mediated ubiquitin-dependent protein catabolic process protein ubiquitination ubiquitin-dependent protein catabolic process Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 64777 66089 Ensembl ENSG00000145916 ENSMUSG00000001054 UniProt Q96G75 Q91YQ7 RefSeq (mRNA) NM_001288794 NM_001288795 NM_022762 NM_025346 RefSeq (protein) NP_001275723 NP_001275724 NP_073599 NP_079622 Location (UCSC) Chr 5: 178.13 – 178.15 Mb Chr 11: 51.51 – 51.53 Mb PubMed search
Wikidata
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Protein sequence

This protein is rich in leucine (14.0%) and might belong to the protein family of leucine-rich repeats

        1 meqcacvere ldkvlqkflt ygqhcersle ellhyvgqlr aelasaalqg tplsatlslv
       61 msqccrkikd tvqklasdhk dihssvsrvg kaidrnfdse icgvvsdavw dareqqqqil
      121 qmaivehlyq qgmlsvaeel cqestlnvdl dfkqpfleln rilealheqd lgpalewavs
      181 hrqrllelns slefklhrlh firllaggpa kqlealsyar hfqpfarlhq reiqvmmgsl
      241 vylrlgleks pychlldssh waeicetftr dacsllglsv esplsvsfas gcvalpvlmn
      301 ikavieqrqc tgvwnhkdel pieielgmkc wyhsvfacpi lrqqtsdsnp piklicghvi
      361 srdalnklin ggklkcpycp meqnpadgkr iif

Homology

text shaded multiple sequence alignment of CAD28476; the shaded amino acids are conserved

CAD28476 is highly conserved in many eukaryotic organism. Its high conservation suggests that it plays a primary role in meiosis.

Orthologs

taxonomic name	common name	NCBI entry	Percentage of sequence similarity	Length (AAs)	comments
Homo sapiens	Human	[1]	100%	393	hypothetical Protein for Meiosis
Pan troglodytes	Chimpanzee	[2]	99.7%	393	required for meiotic nuclear division 5 homolog B isoform 6
Macaca mulatta	Rhesus macaque	[3]	98.5%	393	Similar to CG3295-PA isoform 11
Rattus norvegicus	Norway rat	[4]	98.2%	393	Similar to RIKEN cDNA 0610039K22
Mus musculus	House mouse	[5]	97.7%	393	Required for meiotic nuclear division 5 homolog B
Equus caballus	Horse	[6]	97.7%	273	PREDICTED: similar required for meiotic nuclear division 5 homolog B
Bos taurus	Cattle	[7]	95.4%	393	required for meiotic nuclear division 5 homolog B
Danio rerio	Zebrafish	[8]	72.8%	391	required for meiotic nuclear division 5 homolog B
Xenopus laevis	African clawed frog	[9]	70%	391	MGC84431 protein
Canis lupus familiaris	Dog	[10]	70%	391	PREDICTED: similar to CG3295-PA isoform A
Tetraodon nigroviridis	Spotted green pufferfish	[11]	68.5%	417	unnamed protein product
Ornithorhynchus anatinus	Platypus	[12]	64.5%	389
Branchiostoma floridae	Florida lancelet	[13]	57%	491	hypothetical protein BRAFLDRAFT_126901
Nematostella vectensis	Sea anemone	[14]	50.1%	389
Culex quinquefasciatus	Southern house mosquito	[15]	48.5%	392	conserved hypothetical protein
Aedes aegypti	Yellow fever mosquito	[16]	48.2%	392	hypothetical protein AaeL_AAEL009407
Strongylocentrotus purpuratus	Purple urchin	[17]	48.2	405	PREDICTED: Similar to MGC88921 protein
Drosophila virilis	Fruit fly	[18]	41.5%	437	GJ20343
Drosophila melanogaster	Fruit fly	[19]	40.3%	431	CG3295
Acyrthosiphon pisum	Acrythosiphon	[20]	39.5%	366	PREDICTED: required for meiothic nuclear division 5 homolg A
Oryza sativa	Rice	[21]	32.2%	386	membrane protein-like

Zinc finger domain

This depiction shows the location of the two predicted protein domains.

Result of local sequence alignment- the amino acids found in the zinc finger domain are highlighted in different color

The three amino acids which are coordinated to the zinc ion.

Two domains were predicted by the program BLIMPS to exist in the protein of which one of the domains contains a zinc finger domain.

Zinc finger domains assist the binding of the protein to nucleic acids. This points to a direct interaction of CAD28476 with DNA during meiosis. By comparing CAD28476 with a related zinc finger protein in a local sequence alignment using LALIGN, the amino acids His359, Cys381 and Cys384 could be attributed to the zinc finger domain. This zinc finger structure is uncommon in the way that it involves one histidine instead of two.

Expression

Microarray data show that CAD28476 is highly expressed in tissue where meiosis occurs like in testis and ovaries. Moreover, it is also highly expressed in the brain around the hypothalamus.

Transcriptional regulation

Listed binding sites for different transcription factors, the bold transcription factors are very likely to be involved in regulation.

The analysis of the promoter region (tools on the page rVista . were used) shows that there are several transcription factor binding sites localized in conserved regions . It is very likely that the transcription factor Ets-1 which belongs to the ETS transcription factor family and its core binding factor CBF are involved in regulation of transcription since they both have independent binding sites.

Interacting proteins

There were two proteins predicted which interact with CAD28476.

name of the protein	Uniprot entry	information about the gene
Ewing sarcoma breakpoint region 1, isoform CRA_a	[22]	gene encodes a putative RNA binding protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors.
Mothers against decapentaplegic homolog 4	[23]	SMAD4

Further reading

• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Clark HF, Gurney AL, Abaya E, et al. (2003). "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment". Genome Res. 13 (10): 2265–70. doi:10.1101/gr.1293003. PMC 403697. PMID 12975309.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Colland F, Jacq X, Trouplin V, et al. (2004). "Functional proteomics mapping of a human signaling pathway". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Pope SN, Lee IR (2005). "Yeast two-hybrid identification of prostatic proteins interacting with human sex hormone-binding globulin". J. Steroid Biochem. Mol. Biol. 94 (1–3): 203–8. doi:10.1016/j.jsbmb.2005.01.007. PMID 15862967. S2CID 9746088.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.