RnaG is a small regulatory non-coding RNA encoded by the virulence plasmid of Shigella flexneri, a Gram-negative pathogenic bacterium that causes human bacillary dysentery. It is a first regulatory RNA characterised in S. flexneri. The RNA is 450 nucleotides long (which makes it one of the largest regulatory sRNAs) and it contains a region with specific secondary structure that interacts with icsA (virG) mRNA and forms a transcription terminator. Acting as antisense, RnaG is transcribed from the complementary strand of its target, icsA mRNA.[1] The activity of the incA protein is crucial for spreading of the bacterial pathogen in the host cells.[2]
| RnaG sRNA | |
|---|---|
 Predicted secondary structure and sequence conservation of RnaG small RNA | |
| Identifiers | |
| Rfam | RF02550 |
| Other data | |
| Domain(s) | Bacteria |
| SO | SO:0000077,SO:0000370 |
| PDB structures | PDBe |
References
edit- ^ Giangrossi M, Prosseda G, Tran CN, Brandi A, Colonna B, Falconi M (June 2010). "A novel antisense RNA regulates at transcriptional level the virulence gene icsA of Shigella flexneri". Nucleic Acids Research. 38 (10): 3362–3375. doi:10.1093/nar/gkq025. PMC 2879508. PMID 20129941.
- ^ Bernardini ML, Mounier J, d'Hauteville H, Coquis-Rondon M, Sansonetti PJ (May 1989). "Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin". Proceedings of the National Academy of Sciences of the United States of America. 86 (10): 3867–3871. doi:10.1073/pnas.86.10.3867. PMC 287242. PMID 2542950.