SCIN

Scinderin (also known as adseverin) is a protein that in humans is encoded by the SCIN gene. Scinderin is an actin severing protein belonging to the gelsolin superfamily. It was discovered in Dr. Trifaro's laboratory at the University of Ottawa, Canada. Secretory tissues are rich in scinderin. In these tissues scinderin, a calcium dependent protein, regulates cortical actin networks. Normally secretory vesicles are excluded from release sites on the plasma membrane by the presence of a cortical actin filament network. During cell stimulation, calcium channels open allowing calcium ions to enter the secretory cell. Increase in intracellular calcium activates scinderin with the consequent actin filament severing and local dissociation of actin filament networks. This allows the movement of secretory vesicles to release sites on the plasma membrane.

SCIN
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3FG6, 5A1K, 5A1M
Identifiers
Aliases	SCIN, scinderin
External IDs	OMIM: 613416; MGI: 1306794; HomoloGene: 36296; GeneCards: SCIN; OMA:SCIN - orthologs
Gene location (Human) Chr. Chromosome 7 (human) Band 7p21.3 Start 12,570,577 bp End 12,660,182 bp
Gene location (Mouse) Chr. Chromosome 12 (mouse) Band 12|12 B1 Start 40,109,768 bp End 40,184,227 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in jejunal mucosa tibia renal medulla duodenum Achilles tendon mucosa of transverse colon human kidney buccal mucosa cell palpebral conjunctiva oral cavity Top expressed in Ileal epithelium left colon molar corneal stroma lumbar spinal ganglion Paneth cell gastrula seminal vesicula jejunum skin of external ear More reference expression data BioGPS More reference expression data
Gene ontology Molecular function calcium ion binding phosphatidylserine binding actin filament binding 1-phosphatidylinositol binding actin binding phosphatidylinositol-4,5-bisphosphate binding metal ion binding Cellular component cytoplasm cell cortex brush border extracellular exosome cytoskeleton podosome cell junction cell projection plasma membrane protein-containing complex Biological process sequestering of actin monomers regulation of chondrocyte differentiation actin nucleation actin filament severing positive regulation of actin nucleation positive regulation of secretion actin filament capping positive regulation of apoptotic process positive regulation of megakaryocyte differentiation negative regulation of cell population proliferation calcium-ion regulated exocytosis Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 85477 20259 Ensembl ENSG00000006747 ENSMUSG00000002565 UniProt Q9Y6U3 Q60604 RefSeq (mRNA) NM_001112706 NM_033128 NM_001146196 NM_009132 RefSeq (protein) NP_001106177 NP_149119 NP_001139668 NP_033158 Location (UCSC) Chr 7: 12.57 – 12.66 Mb Chr 12: 40.11 – 40.18 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Further reading

• Rodríguez Del Castillo A, Vitale ML, Trifaró JM (1992). "Ca2+ and pH determine the interaction of chromaffin cell scinderin with phosphatidylserine and phosphatidylinositol 4,5,-biphosphate and its cellular distribution during nicotinic-receptor stimulation and protein kinase C activation". J. Cell Biol. 119 (4): 797–810. doi:10.1083/jcb.119.4.797. PMC 2289683. PMID 1331119.
• Rodríguez Del Castillo A, Vitale ML, Tchakarov L, Trifaró JM (1992). "Human platelets contain scinderin, a Ca(2+)-dependent actin filament-severing protein". Thromb. Haemost. 67 (2): 248–51. doi:10.1055/s-0038-1648420. PMID 1621245. S2CID 20052201.
• Lueck A, Brown D, Kwiatkowski DJ (1999). "The actin-binding proteins adseverin and gelsolin are both highly expressed but differentially localized in kidney and intestine". J. Cell Sci. 111. ( Pt 24) (24): 3633–43. doi:10.1242/jcs.111.24.3633. PMID 9819354.
• Zunino R, Li Q, Rosé SD, et al. (2001). "Expression of scinderin in megakaryoblastic leukemia cells induces differentiation, maturation, and apoptosis with release of plateletlike particles and inhibits proliferation and tumorigenesis". Blood. 98 (7): 2210–9. doi:10.1182/blood.V98.7.2210. hdl:11336/60914. PMID 11568009.
• Nagase T, Kikuno R, Ohara O (2002). "Prediction of the coding sequences of unidentified human genes. XXI. The complete sequences of 60 new cDNA clones from brain which code for large proteins". DNA Res. 8 (4): 179–87. doi:10.1093/dnares/8.4.179. PMID 11572484.
• Lejen T, Pene TD, Rosé SD, Trifaró JM (2002). "The role of different Scinderin domains in the control of F-actin cytoskeleton during exocytosis". Ann. N. Y. Acad. Sci. 971 (1): 248–50. Bibcode:2002NYASA.971..248L. doi:10.1111/j.1749-6632.2002.tb04469.x. PMID 12438125. S2CID 19994226.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Hillier LW, Fulton RS, Fulton LA, et al. (2003). "The DNA sequence of human chromosome 7". Nature. 424 (6945): 157–64. Bibcode:2003Natur.424..157H. doi:10.1038/nature01782. PMID 12853948.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Bouwmeester T, Bauch A, Ruffner H, et al. (2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nat. Cell Biol. 6 (2): 97–105. doi:10.1038/ncb1086. PMID 14743216. S2CID 11683986.
• Ehre C, Rossi AH, Abdullah LH, et al. (2005). "Barrier role of actin filaments in regulated mucin secretion from airway goblet cells". Am. J. Physiol., Cell Physiol. 288 (1): C46–56. doi:10.1152/ajpcell.00397.2004. PMID 15342343.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Otsuki T, Ota T, Nishikawa T, et al. (2007). "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries". DNA Res. 12 (2): 117–26. doi:10.1093/dnares/12.2.117. PMID 16303743.
• Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.