SENP8

Sentrin-specific protease 8 is an enzyme that in humans is encoded by the SENP8 gene.

SENP8
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1XT9, 2BKQ, 2BKR
Identifiers
Aliases	SENP8, DEN1, NEDP1, PRSC2, SUMO/sentrin peptidase family member, NEDD8 specific, SUMO peptidase family member, NEDD8 specific
External IDs	OMIM: 608659; MGI: 1918849; HomoloGene: 14084; GeneCards: SENP8; OMA:SENP8 - orthologs
Gene location (Human) Chr. Chromosome 15 (human) Band 15q23 Start 72,114,258 bp End 72,143,692 bp
Gene location (Mouse) Chr. Chromosome 9 (mouse) Band 9|9 B Start 59,641,542 bp End 59,657,993 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in sperm gonad secondary oocyte testicle right testis left testis muscle of thigh islet of Langerhans gastrocnemius muscle ventricular zone Top expressed in secondary oocyte zygote primary oocyte lumbar spinal ganglion genital tubercle tail of embryo hand otolith organ facial motor nucleus utricle More reference expression data BioGPS More reference expression data
Gene ontology Molecular function peptidase activity cysteine-type peptidase activity hydrolase activity NEDD8-specific protease activity endopeptidase activity Cellular component cytosol nucleus Biological process proteolysis protein deubiquitination protein desumoylation post-translational protein modification Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 123228 71599 Ensembl ENSG00000166192 ENSMUSG00000051705 UniProt Q96LD8 Q9D2Z4 RefSeq (mRNA) NM_001166340 NM_001172109 NM_001172110 NM_001172111 NM_145204 NM_001172068 NM_001172069 NM_001172070 NM_001172071 NM_027838 RefSeq (protein) NP_001159812 NP_001165580 NP_001165581 NP_001165582 NP_660205 NP_001165539 NP_001165540 NP_001165541 NP_001165542 NP_082114 Location (UCSC) Chr 15: 72.11 – 72.14 Mb Chr 9: 59.64 – 59.66 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

NEDD8 (MIM 603171) is a ubiquitin-like protein that becomes conjugated to the cullin (see CUL1; MIM 603134) subunit of several ubiquitin ligases. This conjugation, called neddylation, is required for optimal ubiquitin ligase activity. NEDD8-specific deneddylases, such as NEDP1, or DEN1, are required to process the NEDD8 propeptide at a C-terminal diglycine motif and to remove NEDD8 from cullins (Gan-Erdene et al., 2003).[supplied by OMIM]

Further reading

• Mikolajczyk J, Drag M, Békés M, Cao JT, Ronai Z, Salvesen GS (2007). "Small ubiquitin-related modifier (SUMO)-specific proteases: profiling the specificities and activities of human SENPs". J. Biol. Chem. 282 (36): 26217–24. doi:10.1074/jbc.M702444200. PMID 17591783.
• Drag M, Mikolajczyk J, Krishnakumar IM, Huang Z, Salvesen GS (2008). "Activity profiling of human deSUMOylating enzymes (SENPs) with synthetic substrates suggests an unexpected specificity of two newly characterized members of the family". Biochem. J. 409 (2): 461–9. doi:10.1042/BJ20070940. PMID 17916063. S2CID 12234952.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Wu K, Yamoah K, Dolios G, et al. (2003). "DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1". J. Biol. Chem. 278 (31): 28882–91. doi:10.1074/jbc.M302888200. PMID 12759363.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Xirodimas DP, Saville MK, Bourdon JC, et al. (2004). "Mdm2-mediated NEDD8 conjugation of p53 inhibits its transcriptional activity". Cell. 118 (1): 83–97. doi:10.1016/j.cell.2004.06.016. PMID 15242646.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Reverter D, Wu K, Erdene TG, et al. (2005). "Structure of a complex between Nedd8 and the Ulp/Senp protease family member Den1". J. Mol. Biol. 345 (1): 141–51. doi:10.1016/j.jmb.2004.10.022. PMID 15567417.
• Shen LN, Liu H, Dong C, et al. (2005). "Structural basis of NEDD8 ubiquitin discrimination by the deNEDDylating enzyme NEDP1". EMBO J. 24 (7): 1341–51. doi:10.1038/sj.emboj.7600628. PMC 1142549. PMID 15775960.

External links

• Overview of all the structural information available in the PDB for UniProt: Q96LD8 (Sentrin-specific protease 8) at the PDBe-KB.