SRG1 RNA (SER3 regulatory gene 1) is a non-coding RNA which represses the expression of SER3 (YER081W).[1][2][3] SER3 is a gene which codes for a phosphoglycerate dehydrogenase involved in the biosynthesis of serine.[4] SRG1 represses SER3 expression via transcription interference, and in that respect is the first intergenic transcript of its kind.[1]
| SRG1 | |
|---|---|
 Conserved secondary structure of SRG1 RNA | |
| Identifiers | |
| Symbol | SRG1 |
| Rfam | RF01765 |
| Other data | |
| RNA type | Gene; Cis-reg; |
| Domain(s) | Saccharomyces |
| PDB structures | PDBe |
SRG1 was identified when chromatin immunoprecipitation (ChIP) assays showed that in Saccharomyces cerevisiae, even when SER3 was being repressed, TATA-binding protein and RNA Polymerase II were still bound to the SER3 DNA in such a way that should have caused active transcription.[1] Further analysis found a second TATA box 558 bp upstream of SER3. A bioinformatic scan identified this same TATA box element in related Saccharomyces.[5] SRG1 has been found to be cis-acting, it had no repressive effect when in trans.[1]
SRG1 RNA is unrelated to Senescence Related Gene 1 (SRG1), a protein-coding gene found in Arabidopsis thaliana,[6] and is also distinct from Stress Response Gene 1 (SRG1) found in Medicago sativa.[7][8]
References
edit- ^ a b c d Martens JA, Laprade L, Winston F (June 2004). "Intergenic transcription is required to repress the Saccharomyces cerevisiae SER3 gene". Nature. 429 (6991): 571–574. Bibcode:2004Natur.429..571M. doi:10.1038/nature02538. PMID 15175754. S2CID 809550.
- ^ "Entrez Gene SRG1". 2010-07-03. Retrieved 2010-08-02.
- ^ "Yeast Genome SRG1". Retrieved 2010-08-02.
- ^ Albers E, Laizé V, Blomberg A, Hohmann S, Gustafsson L (March 2003). "Ser3p (Yer081wp) and Ser33p (Yil074cp) are phosphoglycerate dehydrogenases in Saccharomyces cerevisiae". The Journal of Biological Chemistry. 278 (12): 10264–10272. doi:10.1074/jbc.M211692200. PMID 12525494.
- ^ Cliften P, Sudarsanam P, Desikan A, Fulton L, Fulton B, Majors J, Waterston R, Cohen BA, Johnston M (July 2003). "Finding functional features in Saccharomyces genomes by phylogenetic footprinting". Science. 301 (5629): 71–76. Bibcode:2003Sci...301...71C. doi:10.1126/science.1084337. PMID 12775844. S2CID 1305166.
- ^ "Uniprot Knowledgebase - Protein SRG1". Retrieved 2010-08-02.
- ^ Truesdell GM, Dickman MB (March 1997). "Isolation of pathogen/stress-inducible cDNAs from alfalfa by mRNA differential display" (PDF). Plant Molecular Biology. 33 (4): 737–743. doi:10.1023/A:1005728420374. PMID 9132065. S2CID 35519690.
- ^ "GenBank Accs:U42752.1". 27 May 1997. Retrieved 2010-08-02.
Further reading
edit- Cullen BR, Lomedico PT, Ju G (1984). "Transcriptional interference in avian retroviruses—implications for the promoter insertion model of leukaemogenesis". Nature. 307 (5948): 241–245. Bibcode:1984Natur.307..241C. doi:10.1038/307241a0. PMID 6363938. S2CID 4277058.
- Cawley S, Bekiranov S, Ng HH, Kapranov P, Sekinger EA, Kampa D, Piccolboni A, Sementchenko V, Cheng J, Williams AJ, Wheeler R, Wong B, Drenkow J, Yamanaka M, Patel S, Brubaker S, Tammana H, Helt G, Struhl K, Gingeras TR (February 2004). "Unbiased mapping of transcription factor binding sites along human chromosomes 21 and 22 points to widespread regulation of noncoding RNAs". Cell. 116 (4): 499–509. doi:10.1016/S0092-8674(04)00127-8. PMID 14980218.
- Valerius O, Brendel C, Düvel K, Braus GH (June 2002). "Multiple factors prevent transcriptional interference at the yeast ARO4-HIS7 locus". The Journal of Biological Chemistry. 277 (24): 21440–21445. doi:10.1074/jbc.M201841200. PMID 11937506.
- Adhya S, Gottesman M (July 1982). "Promoter occlusion: transcription through a promoter may inhibit its activity". Cell. 29 (3): 939–944. doi:10.1016/0092-8674(82)90456-1. PMID 6217898. S2CID 24587523.
- Hurowitz EH, Brown PO (2003). "Genome-wide analysis of mRNA lengths in Saccharomyces cerevisiae". Genome Biology. 5 (1): R2. doi:10.1186/gb-2003-5-1-r2. PMC 395734. PMID 14709174.