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Strigomonas culicis

Strigomonas culicis is a protist and member of flagellated trypanosomatids. It is an obligate parasite in the gastrointestinal tract of mosquito, and is in turn a host to symbiotic bacteria. It maintains strict mutualistic relationship with the bacteria as a sort of cell organelle (endosymbiont) so that it cannot lead an independent life without the bacteria.[5][6] Along with Angomonas deanei, S. culicis is researched as model organism for the evolution of symbiotic relationsships with intracellular bacteria.

Strigomonas culicis
Strigomonas culicis observed by differential interference contrast (DIC)[1]
Scientific classification Edit this classification
Domain: Eukaryota
Phylum: Euglenozoa
Class: Kinetoplastea
Order: Trypanosomatida
Genus: Strigomonas
Species:
S. culicis
Binomial name
Strigomonas culicis
Teixeira et al., 2011[2]
Type strain
ATCC12982, TCC043E
Synonyms
  • Blastocrithidia culicis Wallace and Johnson, 1961[3]
  • Herpetomonas culicis Novy et al. 1907[4]

Taxonomy

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S. culicis was first described as Trypanosoma (Herpetomonas) culicis in 1907 by Frederick G. Novy,  Ward J. MacNeal, and Harry N. Torreyin 1907.[7] The species name refers to the mosquito genus Culex in which it was found, although it has been found to also be present in other mosquitos such as Aedes.[8] Another description by F.G. Wallace and A. Johnson as Blastocrithidia culicis, published in 1961 based on specimens from Aedes vexans,[3] was later considered synonymous to Herpetomonas culicis. Eventually, by microbiological analysis of endosymbiont harboring Trypanosoma, this species was assigned to the genus Strigomonas in 2011, bearing the current name S. culicis.[2]

The obligate bacterium belongs a group of ß-proteobacterium and provides nutrients to the host, in addition to influencing some of the cellular functions.[9]

Biology

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Stages of dividing Strigomonas culicis with its bacterial symbiont - S (blue in 2 picture) and green indicate the bacterium; K denotes the kinetoplast, and N, the nucleus

S. culicis spends its life cycle in mosquitos. It migrates from the mosquito midgut and enter the body cavity (haemocoel) and finally reside in the salivary glands.[10] Unlike other trypanosomatids, S. culicis does not produce some amino acids such as methionine, histidine, and arginine; and vitamins such as thiamin, nicotinamide, and riboflavin.[8] The bacterium provides these nutrients. In addition, it also provides enzymes required by the host for amino acid synthesis, lipid and purine/pyrimidine metabolism, urea cycle, haeme biosynthesis,[11] protein synthesis, and protein folding. It can not reproduce on its own and relies on signals from the protist's nucleus.[6] Isolated bacteria cannot survive on their own.[11] When the bacteria are removed by antibiotic treatment, the protist survives but can not infect mosquitos.[6]

S. culicis has about 12,162 open reading frames (ORFs).[11]

Symbiont

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The bacterium Ca. Kinetoplastibacterium blastocrithidii is a ß-proteobacterium of the family Alcaligenaceae. It is enclosed in two layers of cell membranes, and unlike typical bacterial membrane, peptidoglycan is greatly reduced.[11] It acts as a cell organelle not only by supplying essential enzymes, but also by replacing paraflagellar rod associated to the axoneme, thus, intimately associated with the kinetoplast.[12] In addition, it provides surplus supply of ATP molecules for increased metabolic activities.[13] During cell division, as the kinetoplast of the host divides so do the bacterium.[14] The host cell controls the number of bacterial division.[1] This coordinated mitosis results in even distribution of one bacterium in each daughter cell.[14]

Ecology

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Strigomonas culicis can be found in several mosquito genera, such as Anopheles, Culex, Aedes[8] and Coquillettidia.

References

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  1. ^ a b Catta-Preta, Carolina M. C.; Brum, Felipe L.; da Silva, Camila C.; Zuma, Aline A.; Elias, Maria C.; de Souza, Wanderley; Schenkman, Sergio; Motta, Maria Cristina M. (2015). "Endosymbiosis in trypanosomatid protozoa: the bacterium division is controlled during the host cell cycle". Frontiers in Microbiology. 6: 520. doi:10.3389/fmicb.2015.00520. PMC 4451579. PMID 26082757.
  2. ^ a b Teixeira, Marta M.G.; Borghesan, Tarcilla C.; Ferreira, Robson C.; Santos, Marcia A.; Takata, Carmen S.A.; Campaner, Marta; Nunes, Vania L.B.; Milder, Regina V.; de Souza, Wanderley; Camargo, Erney P. (2011). "Phylogenetic Validation of the Genera Angomonas and Strigomonas of Trypanosomatids Harboring Bacterial Endosymbionts with the Description of New Species of Trypanosomatids and of Proteobacterial Symbionts". Protist. 162 (3): 503–524. doi:10.1016/j.protis.2011.01.001. PMID 21420905.
  3. ^ a b Wallace, F.G.; Johnson, A. (1961). "The infectivity of old cultured strains of mosquito flagellates". Journal of Insect Pathology. 3: 75–80.
  4. ^ Page Taxonomy browser (Strigomonas culicis) on "Taxonomy Browser". NCBI. Retrieved 2023-12-02.
  5. ^ Chang, K. P. (1974). "Ultrastructure of symbiotic bacteria in normal and antibiotic-treated Blastocrithidia culicis and Crithidia oncopelti". The Journal of Protozoology. 21 (5): 699–707. doi:10.1111/j.1550-7408.1974.tb03733.x. PMID 4217371.
  6. ^ a b c Brunoro, Giselle V.F.; Menna-Barreto, Rubem F.S.; Garcia-Gomes, Aline S.; Boucinha, Carolina; Lima, Diogo B.; Carvalho, Paulo C.; Teixeira-Ferreira, André; Trugilho, Monique R.O.; Perales, Jonas; Schwämmle, Veit; Catanho, Marcos (2019). "Quantitative Proteomic Map of the Trypanosomatid Strigomonas culicis: The Biological Contribution of its Endosymbiotic Bacterium". Protist. 170 (6): 125698. doi:10.1016/j.protis.2019.125698. PMID 31760169. S2CID 208275170.
  7. ^ Novy, F. G.; MacNeal, W. J.; Torrey, H. N. (1907). "The Trypanosomes of Mosquitoes and Other Insects". Journal of Infectious Diseases. 4 (2): 223–276. doi:10.1093/infdis/4.2.223. JSTOR 30072673.
  8. ^ a b c de Menezez, Maria Claudia Noronha Dutra; Roitmanz, Isaac (1991). "Nutritional Requirements of Blastocrithidia culicis , a Trypanosomatid with an Endosymbiont 1". The Journal of Protozoology. 38 (2): 122–123. doi:10.1111/j.1550-7408.1991.tb06030.x.
  9. ^ de Souza, Silvana Sant´Anna; Catta-Preta, Carolina Moura; Alves, João Marcelo P.; Cavalcanti, Danielle P.; Teixeira, Marta M. G.; Camargo, Erney P.; De Souza, Wanderley; Silva, Rosane; Motta, Maria Cristina M. (2017). Yurchenko, Vyacheslav (ed.). "Expanded repertoire of kinetoplast associated proteins and unique mitochondrial DNA arrangement of symbiont-bearing trypanosomatids". PLOS ONE. 12 (11): e0187516. Bibcode:2017PLoSO..1287516D. doi:10.1371/journal.pone.0187516. PMC 5683618. PMID 29131838.
  10. ^ Catta-Preta, C.M.C.; Nascimento, M.T.C.; Garcia, M.C.F.; Saraiva, E.M.; Motta, M.C.M.; Meyer-Fernandes, J.R. (2013). "The presence of a symbiotic bacterium in Strigomonas culicis is related to differential ecto-phosphatase activity and influences the mosquito–protozoa interaction". International Journal for Parasitology. 43 (7): 571–577. doi:10.1016/j.ijpara.2013.02.005. PMID 23562935.
  11. ^ a b c d Motta, Maria Cristina Machado; Martins, Allan Cezar de Azevedo; de Souza, Silvana Sant'Anna; Catta-Preta, Carolina Moura Costa; Silva, Rosane; Klein, Cecilia Coimbra; de Almeida, Luiz Gonzaga Paula; de Lima Cunha, Oberdan; Ciapina, Luciane Prioli; Brocchi, Marcelo; Colabardini, Ana Cristina (2013). "Predicting the proteins of Angomonas deanei, Strigomonas culicis and their respective endosymbionts reveals new aspects of the trypanosomatidae family". PLOS ONE. 8 (4): e60209. Bibcode:2013PLoSO...860209M. doi:10.1371/journal.pone.0060209. PMC 3616161. PMID 23560078.
  12. ^ de Souza, W.; Motta, M. C. (1999). "Endosymbiosis in protozoa of the Trypanosomatidae family". FEMS Microbiology Letters. 173 (1): 1–8. doi:10.1111/j.1574-6968.1999.tb13477.x. PMID 10220875.
  13. ^ Loyola-Machado, Ana Carolina; Azevedo-Martins, Allan Cézar; Catta-Preta, Carolina Moura Costa; de Souza, Wanderley; Galina, Antonio; Motta, Maria Cristina M. (2017). "The Symbiotic Bacterium Fuels the Energy Metabolism of the Host Trypanosomatid Strigomonas culicis". Protist. 168 (2): 253–269. doi:10.1016/j.protis.2017.02.001. PMID 28371652.
  14. ^ a b Brum, Felipe Lopes; Catta-Preta, Carolina Moura Costa; de Souza, Wanderley; Schenkman, Sergio; Elias, Maria Carolina; Motta, Maria Cristina Machado (2014). "Structural characterization of the cell division cycle in Strigomonas culicis, an endosymbiont-bearing trypanosomatid". Microscopy and Microanalysis. 20 (1): 228–237. Bibcode:2014MiMic..20..228B. doi:10.1017/S1431927613013925. PMID 24397934. S2CID 29468549.
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