In molecular biology, TAZ zinc finger (Transcription Adaptor putative Zinc finger) domains are zinc-containing domains found in the homologous transcriptional co-activators CREB-binding protein (CBP) and the P300. CBP and P300 are histone acetyltransferases (EC) that catalyse the reversible acetylation of all four histones in nucleosomes, acting to regulate transcription via chromatin remodelling. These large nuclear proteins interact with numerous transcription factors and viral oncoproteins, including p53 tumour suppressor protein, E1A oncoprotein, MyoD, and GATA-1, and are involved in cell growth, differentiation and apoptosis.[1] Both CBP and P300 have two copies of the TAZ domain, one in the N-terminal region, the other in the C-terminal region. The TAZ1 domain of CBP and P300 forms a complex with CITED2 (CBP/P300-interacting transactivator with ED-rich tail), inhibiting the activity of the hypoxia inducible factor (HIF-1alpha) and thereby attenuating the cellular response to low tissue oxygen concentration.[2] Adaptation to hypoxia is mediated by transactivation of hypoxia-responsive genes by hypoxia-inducible factor-1 (HIF-1) in complex with the CBP and p300 transcriptional coactivators.[3]
| zf-TAZ | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 solution structure of the taz2 domain of the transcriptional adaptor protein cbp | |||||||||
| Identifiers | |||||||||
| Symbol | zf-TAZ | ||||||||
| Pfam | PF02135 | ||||||||
| InterPro | IPR000197 | ||||||||
| SCOP2 | 1f81 / SCOPe / SUPFAM | ||||||||
| |||||||||
The TAZ domain adopts an all-alpha fold with zinc-binding sites in the loops connecting the helices. The TAZ1 domain in P300 and the TAZ2 (CH3) domain in CBP have each been shown to have four amphipathic helices, organised by three zinc-binding clusters with HCCC-type coordination.[4][5][6]
References
edit- ^ Ponting CP, Blake DJ, Davies KE, Kendrick-Jones J, Winder SJ (January 1996). "ZZ and TAZ: new putative zinc fingers in dystrophin and other proteins". Trends Biochem. Sci. 21 (1): 11–13. doi:10.1016/s0968-0004(06)80020-4. PMID 8848831.
- ^ Freedman SJ, Sun ZY, Kung AL, France DS, Wagner G, Eck MJ (July 2003). "Structural basis for negative regulation of hypoxia-inducible factor-1alpha by CITED2". Nat. Struct. Biol. 10 (7): 504–12. doi:10.1038/nsb936. PMID 12778114. S2CID 7652744.
- ^ Freedman SJ, Sun ZY, Poy F, Kung AL, Livingston DM, Wagner G, Eck MJ (April 2002). "Structural basis for recruitment of CBP/p300 by hypoxia-inducible factor-1 alpha". Proc. Natl. Acad. Sci. U.S.A. 99 (8): 5367–72. Bibcode:2002PNAS...99.5367F. doi:10.1073/pnas.082117899. PMC 122775. PMID 11959990.
- ^ De Guzman RN, Liu HY, Martinez-Yamout M, Dyson HJ, Wright PE (October 2000). "Solution structure of the TAZ2 (CH3) domain of the transcriptional adaptor protein CBP". J. Mol. Biol. 303 (2): 243–53. doi:10.1006/jmbi.2000.4141. PMID 11023789.
- ^ De Guzman RN, Martinez-Yamout MA, Dyson HJ, Wright PE (January 2004). "Interaction of the TAZ1 domain of the CREB-binding protein with the activation domain of CITED2: regulation by competition between intrinsically unstructured ligands for non-identical binding sites". J. Biol. Chem. 279 (4): 3042–9. doi:10.1074/jbc.M310348200. PMID 14594809.
- ^ De Guzman RN, Wojciak JM, Martinez-Yamout MA, Dyson HJ, Wright PE (January 2005). "CBP/p300 TAZ1 domain forms a structured scaffold for ligand binding". Biochemistry. 44 (2): 490–7. doi:10.1021/bi048161t. PMID 15641773.