TRNA (adenine58-N1)-methyltransferase (EC 2.1.1.220, tRNA m1A58 methyltransferase, tRNA (m1A58) methyltransferase, TrmI, tRNA (m1A58) Mtase, Rv2118cp, Gcd10p-Gcd14p, Trm61p-Trm6p) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (adenine58-N1)-methyltransferase.[1][2][3] This enzyme catalyses the following chemical reaction
| TRNA (adenine58-N1)-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.1.1.220 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- S-adenosyl-L-methionine + adenine58 in tRNA S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
The enzyme specifically methylates adenine58 in tRNA.
References
edit- ^ Droogmans L, Roovers M, Bujnicki JM, Tricot C, Hartsch T, Stalon V, Grosjean H (April 2003). "Cloning and characterization of tRNA (m1A58) methyltransferase (TrmI) from Thermus thermophilus HB27, a protein required for cell growth at extreme temperatures". Nucleic Acids Research. 31 (8): 2148–56. doi:10.1093/nar/gkg314. PMC 153742. PMID 12682365.
- ^ Varshney U, Ramesh V, Madabushi A, Gaur R, Subramanya HS, RajBhandary UL (2004). "Mycobacterium tuberculosis Rv2118c codes for a single-component homotetrameric m1A58 tRNA methyltransferase". Nucleic Acids Research. 32 (3): 1018–27. doi:10.1093/nar/gkh207. PMC 373377. PMID 14960715.
- ^ Anderson J, Phan L, Hinnebusch AG (May 2000). "The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae". Proceedings of the National Academy of Sciences of the United States of America. 97 (10): 5173–8. doi:10.1073/pnas.090102597. PMC 25801. PMID 10779558.
External links
edit- TRNA+(adenine58-N1)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
