TRNA (carboxymethyluridine34-5-O)-methyltransferase (EC 2.1.1.229, ALKBH8, ABH8, Trm9, tRNA methyltransferase 9) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (carboxymethyluridine34-5-O)-methyltransferase.[1][2][3] This enzyme catalyses the following chemical reaction
| TRNA (carboxymethyluridine34-5-O)-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.1.1.229 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA
The enzyme catalyses the posttranslational modification of uridine residues at the wobble position 34 of the anticodon loop of tRNA.
References
edit- ^ Fu D, Brophy JA, Chan CT, Atmore KA, Begley U, Paules RS, Dedon PC, Begley TJ, Samson LD (May 2010). "Human AlkB homolog ABH8 Is a tRNA methyltransferase required for wobble uridine modification and DNA damage survival". Molecular and Cellular Biology. 30 (10): 2449–59. doi:10.1128/MCB.01604-09. PMC 2863699. PMID 20308323.
- ^ Songe-Møller L, van den Born E, Leihne V, Vågbø CB, Kristoffersen T, Krokan HE, Kirpekar F, Falnes PØ, Klungland A (April 2010). "Mammalian ALKBH8 possesses tRNA methyltransferase activity required for the biogenesis of multiple wobble uridine modifications implicated in translational decoding". Molecular and Cellular Biology. 30 (7): 1814–27. doi:10.1128/MCB.01602-09. PMC 2838068. PMID 20123966.
- ^ Kalhor HR, Clarke S (December 2003). "Novel methyltransferase for modified uridine residues at the wobble position of tRNA". Molecular and Cellular Biology. 23 (24): 9283–92. doi:10.1128/mcb.23.24.9283-9292.2003. PMC 309612. PMID 14645538.
External links
edit- TRNA+(carboxymethyluridine34-5-O)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
