tRNA (guanine37-N1)-methyltransferase (EC 2.1.1.228, TrmD, tRNA (m1G37) methyltransferase, transfer RNA (m1G37) methyltransferase, Trm5p, TRMT5, tRNA-(N1G37) methyltransferase, MJ0883 (gene)) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (guanine37-N1)-methyltransferase.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction
| tRNA (guanine37-N1)-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.1.1.228 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- S-adenosyl-L-methionine + guanine37 in tRNA S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
This enzyme is important for the maintenance of the correct reading frame during translation.
References
edit- ^ Takeda H, Toyooka T, Ikeuchi Y, Yokobori S, Okadome K, Takano F, Oshima T, Suzuki T, Endo Y, Hori H (December 2006). "The substrate specificity of tRNA (m1G37) methyltransferase (TrmD) from Aquifex aeolicus". Genes to Cells. 11 (12): 1353–65. doi:10.1111/j.1365-2443.2006.01022.x. PMID 17121543.
- ^ Lee C, Kramer G, Graham DE, Appling DR (September 2007). "Yeast mitochondrial initiator tRNA is methylated at guanosine 37 by the Trm5-encoded tRNA (guanine-N1-)-methyltransferase". The Journal of Biological Chemistry. 282 (38): 27744–53. doi:10.1074/jbc.m704572200. PMID 17652090.
- ^ O'Dwyer K, Watts JM, Biswas S, Ambrad J, Barber M, Brulé H, Petit C, Holmes DJ, Zalacain M, Holmes WM (April 2004). "Characterization of Streptococcus pneumoniae TrmD, a tRNA methyltransferase essential for growth". Journal of Bacteriology. 186 (8): 2346–54. doi:10.1128/jb.186.8.2346-2354.2004. PMC 412112. PMID 15060037.
- ^ Brulé H, Elliott M, Redlak M, Zehner ZE, Holmes WM (July 2004). "Isolation and characterization of the human tRNA-(N1G37) methyltransferase (TRM5) and comparison to the Escherichia coli TrmD protein". Biochemistry. 43 (28): 9243–55. doi:10.1021/bi049671q. PMID 15248782.
- ^ Goto-Ito S, Ito T, Ishii R, Muto Y, Bessho Y, Yokoyama S (September 2008). "Crystal structure of archaeal tRNA(m(1)G37)methyltransferase aTrm5". Proteins. 72 (4): 1274–89. doi:10.1002/prot.22019. PMID 18384044.
- ^ Ahn HJ, Kim HW, Yoon HJ, Lee BI, Suh SW, Yang JK (June 2003). "Crystal structure of tRNA(m1G37)methyltransferase: insights into tRNA recognition". The EMBO Journal. 22 (11): 2593–603. doi:10.1093/emboj/cdg269. PMC 156765. PMID 12773376.
External links
edit- TRNA+(guanine37-N1)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
