TRNA dimethylallyltransferase (EC 2.5.1.75, tRNA prenyltransferase, MiaA, transfer ribonucleate isopentenyltransferase, Delta2-isopentenyl pyrophosphate:tRNA-Delta2-isopentenyl transferase, Delta2-isopentenyl pyrophosphate:transfer ribonucleic acid Delta2-isopentenyltransferase) is an enzyme with systematic name dimethylallyl-diphosphate: tRNA dimethylallyltransferase.[1][2][3] This enzyme catalyses the following chemical reaction
| TRNA dimethylallyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.5.1.75 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- dimethylallyl diphosphate + tRNA diphosphate + tRNA containing 6-dimethylallyladenosine
Formerly known as tRNA isopentenyltransferase (EC 2.5.1.8), but it is now known that dimethylallyl diphosphate, rather than isopentenyl diphosphate, is the substrate.
Structural studies
editAs of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 2QGN.
References
edit- ^ Leung HC, Chen Y, Winkler ME (May 1997). "Regulation of substrate recognition by the MiaA tRNA prenyltransferase modification enzyme of Escherichia coli K-12". The Journal of Biological Chemistry. 272 (20): 13073–83. doi:10.1074/jbc.272.20.13073. PMID 9148919.
- ^ Soderberg T, Poulter CD (May 2000). "Escherichia coli dimethylallyl diphosphate:tRNA dimethylallyltransferase: essential elements for recognition of tRNA substrates within the anticodon stem-loop". Biochemistry. 39 (21): 6546–53. doi:10.1021/bi992775u. PMID 10828971.
- ^ Moore JA, Mathis JR, Poulter CD (June 2000). "Escherichia coli dimethylallyl diphosphate:tRNA dimethylallyltransferase: pre-steady-state kinetic studies". Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1479 (1–2): 166–74. doi:10.1016/S0167-4838(00)00031-5. PMID 11004538.
Literature
edit- Kline LK, Fittler F, Hall RH (1969). "N6-(Δ2-isopentenyl)adenosine. Biosynthesis in transfer ribonucleic acid in vitro". Biochemistry. 8 (11): 4361–4371. doi:10.1021/bi00839a021. PMID 4311031.
- Rosenbaum N, Gefter ML (1972). "Δ2-Isopentenylpyrophosphate: Transfer Ribonucleic Acid Δ2-Isopentenyltransferase from Escherichia coli. Purification and properties of the enzyme". J. Biol. Chem. 247 (18): 5675–5680. doi:10.1016/S0021-9258(19)44812-6. PMID 4341485.
External links
edit- TRNA+dimethylallyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
