Talk:ADAM7

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I am a student editor for SLU Developmental Biology. Here is a link to my sandbox. This stub needs much more information and I may choose to significantly expand on this information. -Cdxaam

I am flagging this page for my SLU Developmental Biology project. Cdxaam (talk) 20:42, 9 March 2015 (UTC)

Media/Image request

I am attempting to find images to actually use for this article. If any future editors find public crystal data, please upload the image accordingly. Additionally, the article could use a diagram or flowchart depicting the process of membrane transfer (again none seem to be freely available). Cdxaam (talk) 21:57, 12 May 2015 (UTC)

Comments from Cdeclue7 (Peer review)

Hi Cdxaam, I have noted a few improvements that could be done to this article:

1. Consider starting with the anatomical composition of the testes, including the epididymal epithelium that plays a role in the maintenance of the lumen environment that allows sperm maturation (Aumuller, G., Renneberg, H., Schiemann, P.J., Wilhelm, B., Seitz, J., Konrad, L., Wennemuth, G. 1997. The role of apocrine released proteins in the post-testicular regulation of human sperm function. Adv. Exp. Med. Biol. 424: pp. 193-219)
2. Part of this maintenance is the secretion of blebs called epididymosomes, which have been shown to be present in humans (Hermo, L., Jacks, D. 2002. Nature’s ingenuity: bypassing the classical secretory route via apocrine secretion. Mol. Reprod. Dev. 63: pp. 394-410) (Frenette, G., Legare, C., Saez, F., Sullivan, R. 2005. Macrophage migration inhibitory factor in the human epididymis and semen. Mol. Hum. Reprod. 11: pp. 575-582). These structures have been shown to be very important in sperm maturation and mobility (Sullivan, R., Saez, F., Girouard, J., Frenette, G. 2005. Role of exosomes in sperm maturation during the transit along the male reproductive tract. Blood Cells Mol. Dis. 35: pp. 1-10)
3. Consider now transitioning into an overview of the family of ADAMs (Edwards, D.R., Handsley, M.M., Pennington, C.J. 2008. The ADAM metalloproteinases. Mol. Aspects Med. 29: pp. 258-289)(Oh, J., Woo, J.M., Choi, E., Kim, T., Cho, B.N., Park, Z.Y., Kim, Y.C., Kim, D.H., Cho, C. 2005. Molecular, biochemical, and cellular characterization of epididymal ADAMs, ADAM7 and ADAM28. Biochem. Biophys. Res. Commun. 331: pp. 1374-1383)

• I feel the introduction to the article linking ADAM7 to the ADAM family follows the style of the other ADAM family proteins. There is an ADAM family article already existing that I might expand on separately from this article. So directly linking this article with the ADAM article should be sufficient. Cdxaam (talk) 00:34, 22 April 2015 (UTC)

1. This is now when you can introduce the topic of ADAM7, including its expression, distribution, and known functions. You can tie in the aforementioned epididymosomes, as they are the vehicle for interaction between ADAM7 and sperm (Oh, Jeong Su, Cecil Han, and Chunghee Cho. 2009. ADAM7 is associated with epididymosomes and integrated into sperm plasma membrane. Molecules and cells 28(5): 441-446). On the topic of functions, you should consider combining the 2 sections of function that you have made.

• • The first function section was added by another use. It is lacking citation and I am considering how to integrate the information if possible. Sorry for the confusion! Cdxaam (talk) 15:55, 20 April 2015 (UTC)

• • • I attempted to utilize your first few points as best as I saw fit. The article should largely focus on ADAM7, and I feel an in-depth review of the male reproductive tract might be outside the scope of the article. I have linked the key words in the article so that users unfamiliar with the physiology may further investigate the process in detail, while still providing an overview of the process. Cdxaam (talk) 00:30, 22 April 2015 (UTC)

1. Finally, I would consider ending with any known information of ADAM7 (or more generally, ADAM) knockout studies and their findings.

If you increase the content and sources of this article, it should develop into a reliable Wiki article. Have fun! Cdeclue7 (talk) 02:16, 9 April 2015 (UTC)

• • Thank you for the feedback! I will begin to address your points soon. Cdxaam (talk) 23:24, 9 April 2015 (UTC)

Comments from Ayebeesung (Peer review)

• I kind of think the function subheading should be the first subheading, and then everything else.
  • I shall switch this up. The order of all the subheadings is tricky, so this should help the flowCdxaam (talk) 16:55, 29 April 2015 (UTC)
• In the physical characteristics section, you could maybe talk about the structure a little bit more. For example, what about conserved domains across organisms, or domains that contain specific sequences that bind to other proteins, or add another section on this topic alone.
  • Great suggestion. Iw as considering adding more, but did not know what direction to take it. Cdxaam (talk) 16:55, 29 April 2015 (UTC)
• Maybe you could list out other orthologs?
  • The orthologs are actually all listed in the informational links to databases on the article, so I feel this would be unnecessary clutter for a wiki article.Cdxaam (talk) 16:55, 29 April 2015 (UTC)
• Is there a crystal structure of this protein anywhere. Maybe a picture might better orient your wiki viewers.
  • No crystal structure is publicly available it seems. Since its a secreted and membrane bound protein, it probably is challenging to crystallize. Cdxaam (talk) 16:55, 29 April 2015 (UTC)
    • I have been attempting to find some useful picture, but no diagrams are publicly available. All the diagrams are in primary literature so unfortunately I cannot use them without the author's permission (which is not happening anytime soon) Cdxaam (talk) 21:52, 12 May 2015 (UTC)
• Linking "caput epidydymis" could be helpful since most people probably don't know what caput means.
  • The caput epidydymis is described in the epidydymis article itself, so I felt it would be redundant.Cdxaam (talk) 16:55, 29 April 2015 (UTC)

• The first 'sperm' hyperlink should be highlighted, not the second time we see the word 'sperm'
  • Good catch! Cdxaam (talk) 16:55, 29 April 2015 (UTC)

• So is this enzyme only present in males? And what about the other ADAM genes?
  • The enzyme being a key point in spermatogenesis implied its function is only in males. I have not seen anything on females, but I suppose I could add explicit clarification. The other ADAM genes are described in the main ADAM family page. I felt it would be redundant to describe any others since this articles focus is solely on ADAM7.Cdxaam (talk) 16:55, 29 April 2015 (UTC)
• All of the links and citations are working! The format & writing style of this article are very wiki-like too, nice job! Ayebeesung (talk) 16:56, 28 April 2015 (UTC)