Talk:Acid guanidinium thiocyanate-phenol-chloroform extraction

Comment

I am not a fan of wiki's policy on how-tos but this is against the rules, so I copied it here before someone deletes it. read it here (hidden: go to edit or raw mode to retrieve) [1]

About the merge I am not sure about the direction --Squidonius (talk) 13:49 & 13:53, 18 April 2008 --Squidonius (talk) 14:28, 22 April 2008 (UTC)(UTC)

Merged as most editors here are theory people and not methods people. --Squidonius (talk) 23:54, 7 May 2008 (UTC)

• • This article is wrong on several fronts. It's entitled phenol-chloroform_extraction, therefore should present that alone (or have separate section(s) or articles if necessary) for phenol/chloroform extraction, acid-phenol/chloroform extraction, choroform/iaa washing, salt/ethanol(or IPA) precipitation etc.... and yes include

guanidinium-cl/phenol/chloroform extraction too ....but basic phenol/chloroform-extraction as commonly referred to wouldn't include the guanidine as well!! ** — Preceding unsigned comment added by 62.6.248.170 (talk) 11:50, 10 November 2011 (UTC)

This article totally ignores the use of PCIA extraction for DNA purification, where it is frequently indispensable for final preparation for transfection or other procedures sensitive to protein contamination. Also some parts of the article are not consistent with what I has been explained to me about the way the procedure works (eg. I've been told that proteins will crash out at the interphase due to disruption of phenolic rings in the protein, not dissolved in the organic layer). I'll verify some of this stuff and I will probably modify the article to some extent. Vladdybhs (talk) 15:41, 6 August 2008 (UTC)

I mostly agree: the 'How it works' setcion describes procedure for RNA, and is not valid for DNA. At least, the subsection title should be ammended. --AngelHerraez (talk) 11:58, 3 March 2011 (UTC)

I changed: "... and separates rRNA from ribosomes" to "... and separates rRNA from ribosomal proteins".

Furthermore: "... while phenol, isopropanol and water are solvents with poor solubility" --> poor solubility for what? This sentence is unclear.

--Felix Tritschler (talk) 17:17, 8 October 2012 (UTC)

Are you sure about the role of guanidine salts in phenol-based nucleic acid-extraction? This contradicts most protocols for phenol-extraction. Most of these protocols do not use guaninide salts at all. Phenol itself is already a chaotrophic agent. Also, according to most extraction protocols, the separation of RNA and DNA is dependent on the pH of the phenol solution (not any additional chaotrophic salts). At low pH mostly RNA is found in the aqueous phase, at high pH both RNA and DNA are found in the aqueous phase.134.76.70.252 (talk) 16:39, 4 February 2013 (UTC)

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