Talk:Anterograde tracing

Latest comment: 13 years ago by Looie496 in topic Post-mortem
  • Article has been updated, but the introduction may need some expansion: anterograde tracing is meant to label the presynaptic and the postsynaptic neuron(s). The crossing of the synaptic cleft is a vital difference between the anterograde tracers and the dye fillers used for morphological reconstruction. Jhpbroeke (talk) 00:18, 18 February 2010 (UTC)Reply

Post-mortem

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Are there tracing techniques that can be done after the death of the animal? Icek (talk) 17:36, 17 January 2011 (UTC)Reply

Yes, there are a few techniques that can be used in preserved tissue. The main one I know of involves using lipophilic tracers such as diI, which diffuse along axonal tracts even in fixed tissue. The process is very slow, though. Looie496 (talk) 19:07, 17 January 2011 (UTC)Reply

Small changes

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I deleted the reference to Whickersham et al. as their technique concerns retrograde tracing (added in the appropriate article). There were other errors in the statement. Pseudorabies isn't a modified Rabies virus, it's actually a herpes.