Talk:DNA ligase

Wiki Education Foundation-supported course assignment

This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): Nathalieli. Peer reviewers: Nathalieli.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 19:45, 17 January 2022 (UTC)

Wiki Education Foundation-supported course assignment

This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): Axiao12, Joyspark, Ankita5195. Peer reviewers: Joyspark, Sbudhoo11, Ankita5195.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 18:57, 16 January 2022 (UTC)

Merge proposal

A proposal has been made to merge sticky end/blunt end here. My personal reaction is, wouldn't it be more logical to merge into restriction enzyme? // habj 13:27, 8 October 2006 (UTC)

• Yep, that just occured to me. --Peta 01:00, 9 October 2006 (UTC)

A possible reason not to is that restriction enzymes aren't the only things that produce sticky or blunt ends (though restriction enzymes are the most common context, granted). Bryan 09:48, 9 December 2006 (UTC)

Where can i find this guy?

Ntokozo Masina —Preceding unsigned comment added by 196.21.218.142 (talk) 17:17, 14 May 2008 (UTC)

DNA ligase I: connects Okazaki fragments of the lagging strand in DNA replication, and can also seal some repair and recombination fragments (Wei et al., 1995).

so it can ligate single strand breaks, article is pretty unprecise

ATP??

This article says that ATP is not required for a ligase reaction. This is wrong. ATP is required, no energy is derived from the DNA backbone because the 5' phosphate is a mono phosphate nor is it cleaved. I am changing the article. Adenosine^talk 08:24, 17 October 2008 (UTC)

Bacterial DNA Ligase

There are some confusions in the bactieral DNA ligase section of this article. I'll try and sort it out when I get the time. The key bacterial ligases require NAD+. Some also have ATP-dependent ligases, but it's bacteriopages that sometimes have smaller, ATP-dependent ligases. England Expects (talk) 12:49, 31 March 2009 (UTC)

Applications in Molecular Biology Research

The author talks about the necessity of controlling temperature in molecular biology reactions in order to balance the optimal activity of T4 ligase with the Tm of the fragments being ligated. I'm expanding and updating this to include sources. Ksalerno (talk) 17:31, 18 August 2009 (UTC) Kat Salerno

First Sentence

Does the second part of the first sentence contradict the first part of it? It seems to say that ligase repairs single-stranded breaks, then goes on to note that they are actually double-stranded breaks. — Preceding unsigned comment added by 155.52.37.140 (talk) 15:01, 19 July 2011 (UTC)

• I think the first sentence is unclear: "... DNA ligase is a specific type of enzyme, a ligase, (EC 6.5.1.1) that repairs single-stranded discontinuities in double stranded DNA molecules, in simple words strands that have double-strand break (a break in both complementary strands of DNA)." Strands that have double-strand break? I am guessing it means DNA ligase repairs single strand breaks in duplex DNA? I will try to rewrite the sentence for clarity. -Zynwyx (talk) 12:26, 1 June 2012 (UTC)

• On this page (near the bottom) it talks about DNA ligase also repairing duplex DNA that is broken in both strands (non-homologous end joining), on this page it states a DNA ligase is an enzyme which catalyses the formation of phosphodiester bonds specifically at the site of a single-strand break in duplex DNA. So I think there may be some ambiguity between sources as well. — Preceding unsigned comment added by Zynwyx (talk • contribs) 12:44, 1 June 2012 (UTC)

Template?

The article says that Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template. In what sense is the complementary strand used as a template? Polymerase does use the complementary strand as a template when repairing some forms of DNA damage, and that is followed by ligation. Perhaps that is what is meant; as it stands, I believe the article is incorrect. AlphaHelical (talk) 04:05, 30 May 2013 (UTC)