Talk:Differential centrifugation

Untitled

A Paragraph about Velocity Sedimentation should be added.

I think I disagree. :) Actually this article should be shortened and cleaned up. The parts about isopycnic- and ultracentrifugation should be moved to their appropriate articles (see isopycnic centrifugation and ultracentrifuge), and a separate article should be created for rate zonal sedimentation (and merged with Sucrose gradient centrifugation, which is a form of this technique). These are all separate techniques with different applications. I'm not entirely sure what the definition of velocity sedimentation is though (courtesy of the confusing non-consistent centrifugation/sedimentation-terminology, argh...), so if someone else has a better idea... Lvzon (talk) 19:16, 15 September 2008 (UTC)

Merger proposal

The following discussion is closed. Please do not modify it. Subsequent comments should be made in a new section. A summary of the conclusions reached follows.

The result of this discussion was pages merged D O N D E groovily Talk to me 02:17, 18 March 2012 (UTC)

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It looks like about six months have gone by, and the above-mentioned articles about centrifugation techniques are still very much of stub length. The three articles are sufficiently short and closely related to be sections in this one. I'm putting in templates proposing that Sucrose gradient centrifugation, Isopycnic centrifugation, and Equilibrium gradient centrifugation all be merged into this article. As a physical chemist using these techniques for metal nanoparticle synthesis and purification, I might have an atypical perspective, but they all involve spinning something in a density gradient to separate components, right? Vigormaster (talk) 22:10, 29 March 2009 (UTC)

It would be great for the articles to be merged together in a place where the basic method could be discussed. Then links to the more advanced techniques could be posted. That way someone who is looking for more general information can find it easily and for more in depth information they can get it from another source. Also, is it wikipedia style not to capitalize titles. I keep finding pages that should have both words capitalized but they don't. For example: this page is titled Differential centrifugation rather than Differential Centrifugation. --Fapril (talk) 20:40, 22 April 2009 (UTC)

Hi. Thanks for the input. You are absolutely right about the Wikipedia format to capitalize only the first word. I haven't gotten any work done on this merger proposal yet. Maybe later this fall, when my Ph.D. candidacy exam is over, I'll have time to do this. Getting a handle on the universe of Wikipedia articles about centrifugation and the different viewpoints on the subject will be a fairly serious undertaking. Vigormaster (talk) 14:49, 7 August 2009 (UTC)

My two cents: Yes they should be merged, because the applications and theory are similar enough. (I'm a biologist doing viruses and haven't used all the variations personally but I know that sucrose, caesium chloride, and iodixanol ("Optiprep") are all used for virus preps or analysis, for instance). Even if you put all of them in the same article, once redundant/overlapping content is trimmed, the article won't be too long. Xenobiologista (talk) 03:20, 7 April 2011 (UTC)

The discussion above is closed. Please do not modify it. Subsequent comments should be made on the appropriate discussion page. No further edits should be made to this discussion.

Utracentrifugation

"Elongated proteins have larger frictional coefficients, and sediment more slowly to ensure accuracy." I can't work out what this sentence means. Larger/more slowly than what? Who/what does the ensuring? The proteins themselves? At the moment, it reads as if the proteins sediment more slowly with a view to ensuring accuracy. What is really meant? 92.0.61.172 (talk) 08:17, 6 August 2013 (UTC)

Assessment comment

The comment(s) below were originally left at Talk:Differential centrifugation/Comments, and are posted here for posterity. Following several discussions in past years, these subpages are now deprecated. The comments may be irrelevant or outdated; if so, please feel free to remove this section.

No references. I've been searching for some, but can't seem to find any. Help?!--SuperstrainX5 00:41, 16 November 2007 (UTC)

Last edited at 00:41, 16 November 2007 (UTC). Substituted at 13:25, 29 April 2016 (UTC)

Change the title from Differential centrifugation into something more general.

As there are two main forms of sample centrifugation. 1: Differential centrifugation (where the pellet is removed cyclically, until the smallest particles are left) and 2: Density gradient centrifugation (where a pre-formed density gradient is guiding the particles (either isopycnic: where the density of the sample is within that of the gradient, or rate-zonal: where the density of the sample is higher than that of the gradient). Because the articles for many of these terms are redirected to this page, the title cannot be Differential centrifugation, because it only describes a subset of the whole. Therefore, I suggest to edit the title into something like 'Particle centrifugation' or the like. What do you think? I have my terminology backed by this source: https://www.sigmaaldrich.com/technical-documents/articles/biofiles/centrifugation-separations.html Cmkobel2 (talk) 17:16, 21 November 2017 (UTC)