Talk:Fluorescence recovery after photobleaching

Suggestions on possible improvements

This article seem to be a bit biased toward cell biology, but since FRAP is being performed in several other applications, the article should thus be expanded. Also is there little information on the actual diffusion coefficient calculation and the different models existing to perform this (the equation suggested is one of the more crude ones and is more or less only usable in homogene solutions). —Preceding unsigned comment added by Hakkman (talk • contribs) 02:57, 1 February 2008 (UTC)

Assessment comment

The comment(s) below were originally left at Talk:Fluorescence recovery after photobleaching/Comments, and are posted here for posterity. Following several discussions in past years, these subpages are now deprecated. The comments may be irrelevant or outdated; if so, please feel free to remove this section.

The author says that the "lifetime expires". In fluorescence techniques, the term "lifetime" is generally used to refer to the average length of time that a molecule remains in an electronically excited state (as in the stern-volmer quenching constant, Jablonski diagrams, etc). Perhaps the explanation should instead explain that the fluorophores in the photobleached volume are irreversibly(?) changed to a non-fluorescing form. Juanni (talk) 02:13, 14 May 2008 (UTC)

Last edited at 02:13, 14 May 2008 (UTC). Substituted at 15:18, 29 April 2016 (UTC)