Talk:Lysozyme

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Wiki Education Foundation-supported course assignment

This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): Metkyrillos330, Rsig22.

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Wiki Education Foundation-supported course assignment

This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): Gbzwg, Jsolomon580, Mjburke17. Peer reviewers: GomezChristian, TessaColameta, Charles Cole.

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Suggestions

What is the mechanism of action of lysozymes? —Preceding unsigned comment added by 81.23.193.89 (talk • contribs)

Perhaps you should read the article first: "This enzyme functions by attacking peptidoglycan by hydrolyzing the bond that connects N-acetylmuramic acid with carbon four of N-acetylglucosamine." These substances are part of the bacterial cell wall, and lysozyme therefore affects the integrity of the cell wall. JFW | T@lk 16:50, 31 May 2005 (UTC)

I am rather new to editing policies in wikipedia, but I don't think the discussion or talk page is an appropriate venue for asking questions. If you feel the mechanism of action is missing, start a new section on "mechanism" and complain that the explanation is not good enough--Chibibrain (talk) 06:34, 16 November 2009 (UTC)

opsonins

Somebody could define the term "opsonins"? good piece. —Preceding unsigned comment added by 151.199.52.106 (talk • contribs)

molecular data

How about a molecular mass, and isoeclectic point Jasoninkid 15:58, 12 December 2006 (UTC)

Effect of lysozyme on pathogenicity

The connection expressed in the line "Most of the bacteria affected by lysozyme are not pathogenic. In some cases, lysozyme is a primary reason these organisms do not become pathogenic." is not clear. A brief explanation of how lysozyme prevents pathogenicity, or a link to the mechanism, would be usefull. —Preceding unsigned comment added by 212.10.158.247 (talk • contribs) 20:31, 16 January 2007 (UTC)

Industrial use of lysozymes

We need a section that details lysozyme's use in industry and foods - it's a common preservative (E1105) in the grated cheese you can buy on the non-refrigerated aisle in the store. What do you guys think? —Preceding unsigned comment added by 131.111.223.101 (talk) 00:10, 14 May 2008 (UTC)

Doubious statement

Removed from the article:

Lysozyme is commonly referred to as the "body's own antibiotic" since it kills bacteria.

...as the opening sentence of the article. Seems misguided and false, unless, anyone can provide a rationale? 82.16.7.63 21:51, 25 June 2007 (UTC)

My recollection is that that such a statement was already known to be hopelessly simple-minded when I first read about lysozyme in the 1970s. Athel cb (talk) 16:35, 1 September 2022 (UTC)

I am no expert on lysozyme, but the statement about infant formula and bacterial disease seems inconsistant with physiologic processes. Maybe someone should look into finding a credible source or removing it all together. —Preceding unsigned comment added by 209.191.208.165 (talk) 01:40, 10 November 2008 (UTC)

Fungi and yeast

The research showing that the enzyme affects not only bacteria but also yest and fungi has not been mentioned in the article. 173.52.153.51 (talk) —Preceding undated comment added 14:20, 22 July 2009 (UTC).

T4 or other?

This article makes it completely ambiguous as to whether it is referring to T4 lysozyme, or one of its many other variants. To compound this problem, it cites a specific molecular weight and AA length without saying to which lysozyme it belongs. Not to mention there isn't anything in the lysozyme family with a MW of 14.4 kDa. Chicken lysozyme is 14.3 kDa and T4 is considerably more (around 18.7 kDa according to this paper). T4 lysozyme also isn't 129 residues, that's chicken lysozyme. So not only does this article fail to distinguish between the two main types of lysozyme, it also contains information which is incorrect for both types.--129.49.7.125 (talk) 16:09, 30 April 2008 (UTC)

And please don't respond on my talk page, you'll be sending a new message at random to any of the many users of stony brook's library proxy. It represents anywhere from dozens to hundreds of physical machines. --129.49.7.125 (talk) 16:16, 30 April 2008 (UTC)

SigmaAlrdich lists chicken lysozyme as anywhere from 14.6 kDa to 14.7 kDa, while oddly enough a bottle of chicken lysozyme purchased from Sigma lists it as 14.3, either way since this article seems to be talking about chicken lysozyme the 14.4 number is completely wrong.--129.49.7.125 (talk) 16:21, 30 April 2008 (UTC)

According to this article in J. Biochem it's 14.3 kDa. I'd go with a published paper over a chemical supplier.--129.49.7.125 (talk) 16:23, 30 April 2008 (UTC)

Time to disambiguate?

This article is a mixture of information, some of it pertaining to T4, some of it to chicken lysozyme, and the rest to human lysozyme. Would it be worth splitting this into T4 Lysozyme, Chicken Lysozyme, Human Lysozyme, and making Lysozyme into a disambiguation page? This article seems to be relatively low traffic so this discussion seems likely to go unnoticed. Since it's considered within the scope of Wikiproject MCB I'll propose this change there. --129.49.7.125 (talk) 15:19, 1 May 2008 (UTC)

Disagree Well, the policies and guidelines in wikipedia state that it is an encyclopedia to understand stuff and not a dictionary with word for word listing, the example given is acid and base form a single article calle acidity. The article just needs fixing (separate paragraphs (general,T4,chick, human) if you want). The concepts are linked so I strongly disagree with disambiguation. But the article is so short (general, T4, chick, human). btw. Unless you are running a gel its precise weight is not important. it is actually just information and not knowledge (in addition, the glycosylation make it weigh more so the precise number by calculation from sequence is useless) --Squidonius (talk) 21:11 + correction, 1 May 2008 (UTC)

Which is exactly why I removed the assertion of a specific molar mass.--129.49.7.125 (talk) 17:05, 2 May 2008 (UTC)

The only this I dislike on the article are

• Most of the bacteria affected by lysozyme are not pathogenic... Because Most bacteria are commensal in westerners!
• they developed penicillin, which Fleming had failed to do. that sentence is so odd I donno what it is about; yes, it took twenty years to develop penicillin but I though people at Merk did it. --Squidonius (talk) 00:18, 3 May 2008 (UTC)

PDB structure

Which pdb structure is the best representative of lysozyme? Is 133l just alphabetically the first in the list? —Preceding unsigned comment added by 129.78.64.102 (talk) 00:23, 17 April 2009 (UTC)

133l​ is a reasonably good structure (Res = 1.8 Å). There are higher resolution structures available (e.g., 1jsf​ Res = 1.1 Å), but given the the figure does not display side chains and the figure itself is relatively low resolution (500 × 500 pixels), I don't think it would matter. At the resolution of the figure shown in this article, all the lysozyme crystal structures would look virtually identical. Cheers. Boghog2 (talk) 16:03, 22 July 2009 (UTC)

Coming soon will be a gallery of up to thirty structures added to each of these Gene Wiki articles including this lysozyme article. Boghog2 (talk) 06:30, 23 July 2009 (UTC)

generic stuff human protein

all articles human proteins should include net charge, mol weight, +- carbohydrate, etc cinnamoncolbert —Preceding unsigned comment added by 65.220.64.105 (talk) 14:25, 8 September 2009 (UTC)

RCSB PDB protein database structures

The infobox is taking too much space compared to the text. It seems pedantic to enumerate every PDB structure for lysozymes. It may be better to just include the most important ones, that is, most relevant for human or mammal biochemistry. The reader, if interested, may then navigate to a complete list of RCSB PDB structures, contained in its own article, if this type of list making is tolerated on wikipedia.--Chibibrain (talk) 06:45, 16 November 2009 (UTC)

To consider for inclusion

UCI team discovers how protein in teardrops annihilates harmful bacteria (press release), UC Irvine, 19 Jan 2012, retrieved 22 Jan 2012, It turns out that lysozymes have jaws that latch on and chomp through rows of cell walls like someone hungrily devouring an ear of corn, according to findings that will be published Jan. 20 in the journal Science.

More detailed therapy

The article seems to be in lack of some specific examples or application in therapy of Lysome--Gbzwg (talk) 05:05, 23 February 2017 (UTC)

Significance of lysozyme

It will be helpful if some facts about significance of lysozyme is added.--Gbzwg (talk) 05:07, 23 February 2017 (UTC)

Comments

What is the history of the mechanism? What is the evidence supporting one mechanism over another?--Jsolomon580 (talk) 03:12, 28 February 2017 (UTC)

possible plagiarism detected

Hey, I noticed that the first line of this article is "These are enzymes that damage bacterial cell walls by catalyzing hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins."

And found this in this Cell biochemistry and Biophysics Paper https://link.springer.com/article/10.1007%2Fs12013-012-9485-4

"Lysozyme is a family of enzymes which damage bacterial cell walls by catalyzing hydrolysis of 1,4-b-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-Dglucosamine residues in chitodextrins."

Only difference is that "lysozyme is a family of enzymes which" is change to "These are enzymes that" Mjburke17 (talk) 04:33, 28 February 2017 (UTC)

The text in question was added to Wikipedia in September of 2007 while the source you provided was published six years later. However there is an even older source (copyright 2006) that proceeds both. It would be difficult to paraphrase the sentence, because it is so simple. Will work on this later when I have time. Boghog (talk) 05:02, 28 February 2017 (UTC)

Even older source from 1994. Boghog (talk) 05:31, 28 February 2017 (UTC)

Full citation: Manchenko GP (1994). "Lysozyme". Handbook of detection of enzymes on electrophoretic gels. Boca Raton, Fla.: CRC Press. p. 223. ISBN 978-0-8493-8935-1.

Boghog (talk) 05:31, 28 February 2017 (UTC)