Talk:Nanopore sequencing

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This article was the subject of a Wiki Education Foundation-supported course assignment, between 4 September 2019 and 4 December 2019. Further details are available on the course page. Student editor(s): VmillerB.

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Wiki Education Foundation-supported course assignment

This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): Bryochemist.

Above undated message substituted from Template:Dashboard.wikiedu.org assignment by PrimeBOT (talk) 04:50, 17 January 2022 (UTC)

Comment

Other methods exist to create a non-biological nanopore. Ion-beam sculpting is not the only method, see nanopore

I emailed oxford and suggested (in summary) using helicase modified with symmetric binding sites for alpha-hemolysin (maybe engineered using antibodies to subunits?), guaranteeing a centered fit. Free ATP + DnaC, DnaC reduction (prevent unloading), then more ATP would then, hopefully, move ssDNA through the pore via helicase's sliding action. Personally, I think this technology is critical. 98.122.161.101 (talk) 15:25, 27 June 2010 (UTC)

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Major Revisions

I have just published a majority of the edits that I have made for this page throughout the last month or so. I found the page, before I modified it, to substantially lack any sort of logical layout of the sections. I found this to be important because nanopore systems can be categorized into one of two different groups based on type, biological or solid state (except a hybrid of the two- though, these systems are far less numerous right now). Concurrently, I added a section describing the pros and cons of the two types of nanopore sequencing systems. I have not added a subsection for solid state systems under this aforementioned section yet but I plan to do so at some point. I also added some information that I found to be pertinent to nanopore sequencing such as the theoretical way that detection and identification is achieved by nanopore sequencing systems. As well, I removed some text if I considered it to be incorrect, misleading, or inappropriate for this page. There is definitely more information that could be added and should be added- the first being any other types of nanopore sequencing systems that have not been included. I will most likely add to this page going forward so consider what I have changed so far to, hopefully, be a better layout and structure of the information that was already contained in the page. Please let me know if you have any questions, comments or would simply like to discuss any of this in more detail, thanks.

(Bryochemist (talk) 19:27, 20 April 2017 (UTC))

Applications

Looking at the line "Publications on the method outline its use in rapid identification of viral pathogens,[2] monitoring ebola,[3] environmental monitoring,[4] food safety monitoring, human genome sequencing,[5] plant genome sequencing,[6] monitoring of antibiotic resistance,[7] haplotyping[8] and other applications.";

This seems a little misleading b/c in theory any sequencing technology could be used for those applications. Why offer a general list of things that DNA sequencing can do if this is an article about one very specific DNA sequencing technology? NickCT (talk) 17:42, 8 February 2018 (UTC)

Hi NickCT - you have a point here but there is a rationale - nanopore sequencing does have different properties from other technologies, most noteably for these examples real time data streaming and portability, which have been cited in multiple publications including in this page. eg rapid ID of pathogens is possible because of the real time availability of the data, which can result in an ID in hours for a whole workflow. This could be useful to make in the text? Uk2008gen (talk) 13:38, 9 April 2018 (UTC)