Talk:pUC19

Untitled

this article is about the vector pUC19 and has been written coherently. i have aslo included the DNA sequence of the plasmid for this article. i have also included two references that can be verified. it will be beneficial for the readers if it is not deleted. i am ready to answer any further questions raised regarding the article. Proquence (talk) 18:10, 19 November 2008 (UTC)

Might it be a good idea to provide the GenBank accessions for the plasmids?

pUC18 L08752

pUC8 L08959

pUC19 M77789

pUC9 L09128

There are probably more.

I won't add these to the article, I'd rather leave that to those more wiki knowledgeable than me.

Also, there were similar pEMBL vectors back in the day, they might rate a mention. JohnMc (talk) 15:05, 30 September 2024 (UTC)

The speedy deletion might be due to the large DNA sequence data i have added. please delete the notice if no one has any objection. Proquence (talk) 18:21, 19 November 2008 (UTC)

The article in no way ever qualified for speedy deletion, and, as reviewing administrator, I removed the tag. I see nothing wrong with having the sequence. What the article needs is, first, full citations in the usual academic style to its preparation and analysis, preferably from formally published sources, not just web sites, and, a few selected references from other publications to its use and importance. Include the authors, the title of the paper, the name of journal and the publication information. Also includes a suitable web site where the paper or at least an abstract cna be found, and the PubMed ID. -- all according to WP:CITE. DGG (talk) 19:02, 19 November 2008 (UTC)

Can the name of the article be modified to pUC19, instead of PUC19? Proquence (talk) 13:49, 24 November 2008 (UTC)  Done waggers (talk) 13:56, 24 November 2008 (UTC)

Incorrect citation

Mooreland; et al. (2013). Advanced Biomolecular Genetics. Kleiske Publishing. pp. 889–932. Cannot verify the existence of this citation. Kleiske Publishing does not seem to exist. Citation should be removed or replaced. --72.204.6.233 (talk) 03:55, 17 March 2022 (UTC)

pUC19 versus other pUC vectors

The article briefly mentions pUC18 and compares pUC19 with it. Now, I came to wikipedia to find out how many pUC variants exist, historically. I only know of pUC18 and pUC19, but not why they were named that way, and how many other pUC variants may exist. Would it be possible to add a new article at wikipedia, that can refer to ALL these pUC variants? It is ok if there is only pUC18 and pUC19, but it would be nice if that can be mentioned in a separate article clearly. (Mentioning it here in the pUC19 article may not be 100% appropriate, if there are many pUC-variants. Right now I simply don't know how many variants exist, e. g. if there are more than 2 variants or not.) 2A02:8388:1643:D680:C23D:D743:2A9C:ACFE (talk) 21:09, 27 July 2024 (UTC)

There was at least also pUC8 and pUC9. They're referenced in Vieira and Messing, Gene Vol 19, #3, Oct 1982, Pages 259-268

Basically, the Messing lab made a bunch of M13 phage derivatives for cloning, the M13mp series, and moved the lacZ alpha and the different multiple cloning sites into the pUC plasmids.

The pUC version got its number from its parent M13mp series number, for example, pUC8 was derived from M13mp8, pUC18 from M13mp18, and so on.

I've never really looked at how many, but there were a bunch made back in the '80s.

It's a little difficult because most of the references aren't free to access. If you're at a university or at a company which has journal access, that's the way to go.

In any case, one could make a list of all the M13mp series and their matching pUC daughters from the series of papers relating to them from the Messing lab, given the time and journal access. JohnMc (talk) 14:52, 30 September 2024 (UTC)