Talk:RAR-related orphan receptor gamma

Expansion of RORgamma article

Hello,

I have just made extensive changes to this article: new sections, expanded text, bibliography.

Regards,

--Nicodetr (talk) 02:09, 25 October 2008 (UTC)

Hi. Thank you for your great additions! I have expanded the article somewhat further, particularly to address the question of whether RORγ1 protein is expressed. The general consensus in the literature is that it is not. This is somewhat surprising given the high RORγ1 mRNA expression levels. Since as you point out, the regulation of RORγ1 appears to be linked to circadian rhythm, it is possible that researchers have not looked at the right time of day. It is also possible that RORγ1 protein might be expressed in stem cells (e.g., muscle stem cells) and not in their fully differentiated progeny, and therefore would be difficult to detect given the comparatively small number of stem cells in any tissue. Any thoughts? Cheers. Boghog2 (talk) 15:44, 25 October 2008 (UTC)

Hello. Thanks for your further work on the text. As for the way the isoforms are produced, it is not by differing polyadenylation signals, but on the contrary, from selection of different promoters, which lead to different N-terminal moiety in the protein. I had put the right references that say that in my previous version. Also, I wouldn't say that the expression is particularly high in the muscle. I work on this gene (I mean, in my job in real life!) and in the muscle we see somewhat lower amounts in muscle than in the liver (at peak levels), for instance. As you say, one has to be cautious with the time of analysis. Thus, I would remove the note on low protein levels. Although nobody has checked directly, there are evidence that in the KO mice, the expression target genes of RORgamma is reduced (e.g. Arntl/Bmal1, p21, Hsp60...), implying that this isoform is produced at the protein level and active. For this same reason, I would also remove the phrase that no other phenotype than immune is known. Circadian phenotype are known, and it is that mainly immune system has been checked up to now. Also, after your changes, sometimes you say gamma1 and 2, sometimes gamma and gammaT. We should be consistent; and the more widespread nomenclature in the literature is the latter (gamma and gammaT). I can make those changes, but I do not have time today, so I may do them on another day if needed. Regards, --Nicodetr (talk) 23:46, 25 October 2008 (UTC)

Hello Boghog2. We made changes at the same time; I have tried to merge our changes. Note that I removed the polyadenylation part. Given the structure of the two mRNA isoforms, it is impossible that they are due to alternative polyadenylation signals. It must be selection of alternative promoters. We and others have published data in this sense, and the gene structure also goes along this. Regards, --Nicodetr (talk) 13:12, 26 October 2008 (UTC)

Thanks for your additional edits and sorry about the edit conflict. I think we have come to a good compromise. Concerning the lack of an altered RORg-/- circadian rhythm phenotype, the Liu 2008 manuscript suggests it may be due to functional redundancy with RORa. Cheers. Boghog2 (talk) 14:54, 26 October 2008 (UTC)

Hello. Functional redundancy of RORa and RORg (as well as the opposite effect of rev-erbs on Bmal1 during the day) causes the not-so-serious phenotype of each of the mutations. Thanks for the collaboration on this article. Also, thanks for proof-reading my still-imperfect Wikipedia syntax! I'll probably work on the ROR page one of these days. Regards, --Nicodetr (talk) 18:35, 26 October 2008 (UTC)

Likewise, thank you for your great additions. This article has come a long way in a short time. In case you haven't already seen this, you might be interested in User:Diberri's Wikipedia template filling tool. Given a PubMed ID, this tool generates a filled in Wikipedia in-line citation template which you can copy and paste into a Wikipedia article. Be sure to turn on the "add ref tag" option. Cheers. Boghog2 (talk) 19:48, 26 October 2008 (UTC)

I have been using this tool, but without the ref tag tool. Thanks for the tip! --Nicodetr (talk) 22:27, 26 October 2008 (UTC)

Tissues expressing RORgT

The article says that RORgT is restricted to the thymus, but then says it is found in LTi cells and Th17s. As these cells are present in tissues other than the thymus, surely this means RORgT is not restricted to the thymus? 144.32.126.12 (talk) 17:04, 12 December 2010 (UTC)