Talk:RNA splicing

Untitled

This article needs a considerable amount of expansion, as it is a very important area of cell biology. Also, I move that it be moved to splicing (molecular biology), as genetics usually refers to the mode of inheritance, not the process of gene expression. A disambiguation page should be created for splicing, as it also refers to (to my memory) something to do with the manufacture of ropes. I'll try to make some edits when I have the time and have revised the subject.--ChrisJMoor 02:50, 3 Mar 2005 (UTC)

I agree about the need of expansion and, imho, alternative splicing could be merged with this article (unless you're going to really add a lot of new stuff here). However I'm not so sure about renaming this article because genetics, biochemistry and molecular biology are such overlapping terms. --EnSamulili 20:24, 11 Mar 2005 (UTC)

This is very true, Ensamulili. I'll leave the article in its place. Not sure about the merge issue as I was planning to expand it some when I have the time and have revised the subject some.

I also agree. I’m new, but I would like to help. To add to the list of problems with this page, it seems to contain a good deal of information amount mRNA processing that is not actually splicing, such as addition of the cap and the poly-A tail. These topics seem to have crept into earlier versions where it was claimed that the spliceosome made these additions. While that factual error has been corrected, the presence of these additional topics have remained. I wonder if a page about mRNA processing in general would be a more appropriate place for this information.--Plociam 23:22, 20 July 2005 (UTC)

Expanded Sections

I've expanded the article, introducing sections on splicing pathways, evolution, mechanism, and splicing errors. I've also added a section on alternative splicing which refers to the main article; this could be used for a merge in the future, but should probably remain as is now that splicing (genetics) is expanded. I've also added the section splicing (genetics)#Splicing errors for future expansion concerning the kinds of errors that can occur and the diseases they cause.

I'd like to move for a removal of the last section concerning mRNA structure and processing as I feel that it is off-topic. This section is described in the mRNA article, where it is probably more appropriate. --Plociam 00:54, 26 July 2005 (UTC)

I agree that there must be expansion of sections on this page, particularly that of Self-Splicing under Splicing Pathways. The subsection has a misleading introductory sentence for individuals who might be looking for information on this topic because it implies that there will be a discussion of all 3 groups of splicing. However, there is (1) no discussion of Group III intron splicing and (2) no citations in this section for even the short discussion on Group I and II. Nmneuro (talk) 06:51, 1 April 2023 (UTC)NMNeuro, 01 April 2023

F

Proposed Added Link

Experimental manipulation of splicing using Morpholino antisense oligos has become a common technique in developmental biology. Splicing can be blocked by targeting a Morpholino to the snRNP binding sites at the donor and receptor sites, or by targeting the nucleophilic adenine. As I represent the company that manufactures Morpholino antisense, I feel it is better to leave the decision regarding linking the splicing page to the Morpholino article up to a neutral third party. Here is a reference to the original splice study using Morpholinos in zebrafish: [1] The paper is available online: http://www.gene-tools.com/files/draper_etal.pdf There have been hundreds of subsequent papers using Morpholino-mediated splice modification. The technique works in embryos and in tissue culture. jmoulton@REMOVETHISgene-tools.com

23 Jan 06 As there has been no discussion of antisense manipulation of splicing, I have added a section on experimental manipulation of splicing. I have also made the previous reference callout a live link to PubMed and added additional references. Jon

A related discusion can be found at Talk:Influenza#Verifyability.2C_understandability.2C_relevance_concerns. WAS 4.250 23:42, 24 March 2006 (UTC)

Snurps

I readded that snRNP are pronounced Snurps, as that is how they are commonly referred to in medicine and research. Rrten00 19:50, 14 December 2006 (UTC)

Noncanonical splicing; reference problem

Added explanations for canonical (major spliceosome) and noncanonical (minor spliceosome) splicing. Unfortunately I had to renumber all references manually, since I had to add one at the beginning. Maybe an auto-numbered reference template should be used instead. Tekkaman 12:32, 24 January 2007 (UTC)

Fixed it. Samulili 18:15, 24 January 2007 (UTC)

contradiction?

Although the two splicing mechanisms described below do not require any proteins to occur, 5 additional RNA molecules and over 50 proteins are used and hydrolyzes many ATP molecules.

129.31.72.52 22:45, 30 May 2007 (UTC)

While it is true that self-splicing can occur without the addition of proteins, the proteins significantly speed up the process. I agree that the phrasing in the article is confusing and should be clarified.

Aal42 (talk) 14:32, 4 April 2011 (UTC)

The article currently says, "Since in prokaryotic genomes introns do not exist, splicing naturally only occurs in eukaryotes." and "Splicing occurs in all the kingdoms or domains of life....Prokaryotes......splice rarely, but mostly non-coding RNAs." The Group II intron article correctly says that Group II introns are found in bacteria. In The origin of introns and their role in eukaryogenesis: a compromise solution to the introns-early versus introns-late debate?, Koonin suggested that spliceosomal introns originated from group II introns following an endosymbiotic event. --JWSchmidt 03:32, 1 September 2007 (UTC)

Discarded introns

Since my edit comment was a bit unclear: "However, regardless of which pathway is used, the excised introns are discarded." was removed because some microRNAs and other non-coding RNAs come from mRNA introns. Narayanese (talk) 21:41, 27 May 2008 (UTC)

Minor spliceosome

I'd like to propose that the following sentence be changed: "Like the major spliceosome, it is only found in the nucleus.[5]" Studies published in 2005 have shown that splicing can occur in dendrites and in human anucleate platelets. Also, the minor spliceosome in particular has been shown to be predominantly located in the cytoplasm in at least one study I am aware of (König et al. 2007, Cell). Darielab (talk) 00:13, 4 July 2009 (UTC)

I just saw the section on the minor spliceosome mentions this: "The location of spliceosomal activity for the minor class spliceosome is regarded by most experts to be in the nucleus. However, a single paper has claimed that the minor spliceosome is active in the cytosol[7]. The data presented within this paper are not fully accepted within the field and directly contradict numerous other papers, both past and present." Perhaps the sentence on this page could be changed to something similar. Darielab (talk) 00:21, 4 July 2009 (UTC)

external links

external links available on this site:

http://highered.mcgraw-hill.com/sites/dl/free/0072437316/120060/ravenanimation.html

under chapter 15. how splicesosomes process RNA

i don't know how to add it. —Preceding unsigned comment added by Sameerbau (talk • contribs) 07:43, 11 October 2009 (UTC)

Figures

It would be nice to see a couple of figures that show the steps being described in "Self-splicing" and "Spliceosome formation and activity DANK FUCKING MEMES." Aal42 (talk) 14:37, 4 April 2011 (UTC)

I find the Figure (http://en.wikipedia.org/wiki/RNA_splicing#mediaviewer/File:0326_Splicing.jpg) confusing. There are two intronic regions flanking exon2 in the pre-spliced RNA, then in the spliceosome-bound RNA, the right intronic region has just disappeared. 67.207.123.154 (talk) 19:12, 11 November 2014 (UTC)

Too technical?

I don't want to remove the banner myself, but I don't think it should be there. I'm a freshman taking biology in high school, which hardly qualifies as "expert", and I got 90% of what this page what trying to get across.

article is helpful and the resources are reliable.--RawansAljohani (talk) 03:51, 11 September 2018 (UTC)

I agree, it already seems pretty simple compared to the actual subjects level. A simpler version can be on Simple English Wikipedia. Benj5378 (talk) 15:45, 13 June 2022 (UTC)

Wiki Education assignment: CHEM 378 - Biochemistry Lab - spring 2023

This article was the subject of a Wiki Education Foundation-supported course assignment, between 30 January 2023 and 20 May 2023. Further details are available on the course page. Student editor(s): R210240 (article contribs).

— Assignment last updated by R210240 (talk) 02:54, 16 May 2023 (UTC)