Talk:Reduced representation bisulfite sequencing
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Orphan Link
editThis technique is an evolution of the older technique where we use sodium bilsufite to modify unprotected Cytosines into Uracil (Cytosines can be protected with Methyl-groups). This technique was developed in 2009, I think. See this link to the scientific paper: Smith, Z. D., Gu, H., Bock, C., Gnirke, A. & Meissner, A. High-throughput bisulfite sequencing in mammalian genomes. Methods 48, 226–232 (2009).
I am however unsure where to find the bisulfite technique... 84.112.136.52 (talk) 14:48, 2 November 2013 (UTC) Edit: Found it, linked to it http://en.wikipedia.org/wiki/Bisulfite_sequencing - not sure if my formatting is proper, but at least the content is valid IMO. I also removed the orphan-notice because we now have a link to the "parent" technique in the article. 84.112.136.52 (talk) 14:51, 2 November 2013 (UTC)
MspI cut site
editThe cut site of MspI given here is wrong. It is 5'CCGG3', not 3'CCGG5'. See New England Biolabs MspI page. — Preceding unsigned comment added by Partridgefoot (talk • contribs) 17:11, 1 April 2014 (UTC)