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A suitable reference for the Edit under Incubator Shaker
which reads "Anyone employing an incubator shaker (thermal shaker) to grow yeast or bacteria in the laboratory needs to beware that under the usual conditions encountered in the lab, the rate at which oxygen diffuses from the gaseous phase into the shaken liquid phase is too slow to keep up with the rate at which the oxygen is consumed by for example E coli dividing every half hour or S cerevisiae dividing every hour. If the investigator measure the oxygen in the shake flask on the shaker -- polarographically, for example -- at mid-exponential phase of growth, the dissolved oxygen concentration will turn out to be zero."
is Figure 4 of
Eng. Life Sci. 2014 00, 1-10 Engineering in Life Sciences =
Noninvasive online biomass detector system for cultivation in shake flasks
Jörg Schmidt‐Hager Christian Ude Michael Findeis Gernot T. John Thomas Scheper Sascha Beutel
First published: 27 June 2014 https://doi.org/10.1002/elsc.201400026
Appearing in Issue 5 June 27 2014 Richard8081 (talk) 22:45, 13 December 2019 (UTC) — Preceding unsigned comment added by Richard8081 (talk • contribs) 22:41, 13 December 2019 (UTC)
Unfortunately that reference from Eng Life Sci is behind a paywall. Richard8081 (talk) 22:45, 13 December 2019 (UTC)Richard8081 (talk) 01:48, 26 December 2019 (UTC)Reply