Talk:Transcription-mediated amplification

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TMA means transcription mediated amplification. Here the target is RNA amplification. A primer is used to convert RNA into ds DNA using reversed transcriptase,the ds DNA is then converted into RNA amplicons using RNA polymerase, which are again converted to ds DNA for further production of more RNA amplicons. In about 15 min, between 100-1000 RNA can be produced. this whole process is isothermal amplification of RNA by TMA. by Onoja Mike Anthony

"This biochemistry article"...

Latest comment: 3 years ago2 comments2 people in discussion

Just a gentle reminder that this is not a biochemistry article but pertains to Molecular Biology. Calling this article too technical is not appropriate. This subject matter requires a certain amount of background knowledge in DNA, RNA and the enzymes involved in transcription. This will be a long article indeed if you had to add a whole chapter on these subjects. If the interest is there you can work your way backwards to genetics and the process of protein synthesis starting from a region on a DNA strand. — Preceding unsigned comment added by 2A00:23C7:840C:A700:84E1:98DA:7EA5:190F (talk) 19:51, 20 August 2020 (UTC)Reply

Perhaps, but the article is brief and seems to have plenty of room for enlargement. It would seem much more could be explained with few words. Preceding the first use of "ds" with "double-stranded" would help.

It is unclear to me where the multiplication takes place. I can guess that a single RNA molecule (avoiding "strand" here) is reverse-transcribed repeatedly to DNA by an RT, that DNA is transcribed 1:1 to RNA, and the cycle begins again. Does this happen in stages - reverse transcription (to some stopping point) followed by transcription (to some stopping point) - or are both RT and T going on simultaneously? And perhaps there might be a pointer to something explaining why one is multiply transcribed, but not the other (if that's the case)? Apologies in advance if I did not execute this comment correctly. I did try to follow instructions. FWIW, i came here because a CDC page lists "some of" the amplification mechanisms represented by "NAAT", with little explanation of any of them. --Captain Puget (talk) 21:22, 25 August 2021 (UTC)Reply

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