Talk:Transposon sequencing

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	This article was accepted on 4 June 2014 by reviewer LukeSurl (talk · contribs).

Molecular Biology: Genetics / MCB

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Some comments on the lede

Latest comment: 10 years ago2 comments2 people in discussion

(These comments are copied from Wikipedia_talk:WikiProject_Genetics#AfC_submission_-_26.2F05.)

From the first paragraph: "In fact, when the transposon inserts itself in the gene, the gene's function will be disrupted." Which transposoon? Which gene? I wonder if it means "In fact, when a transposon inserts itself in a gene, the gene's function will be disrupted."
Second paragraph: "Transposon sequencing (Tn-seq) is a type of transposon insertion sequencing." But presumably, transposon insertion sequencing is a type of transposon sequencing, making this definition circular, or perhaps redundant. And if the reader is expected to understand "transposon insertion sequencing", it ought to link to an explanation.
Third paragraph: "next generation sequencing". This sounds like marketing language. If it isn't, it needs to be defined (and it would be better to use a less ephemeral term, if one exists).
Third paragraph again: "our knowledge of gene function remains the limiting factor in our understanding of the role genes play." This seems to mean ""our knowledge of what genes do remains the limiting factor in our understanding what genes do." I hope it can be made clearer.

Maproom (talk) 07:55, 3 June 2014 (UTC)Reply

• Citations for related transposon insertion sequencing methods are absent. • The "Tn-Seq" paper discussed is not the first reported application of transposon insertion sequencing. •Some of the other citations are incomplete. — Preceding unsigned comment added by 192.231.71.2 (talk) 20:04, 9 October 2014 (UTC)Reply

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