| Pos | Team | Pld | W | L | D | GF | GA | GD | Pts | PPG | Qualification |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Greenville Triumph SC | 16 | 11 | 3 | 2 | 24 | 11 | +13 | 35 | 2.19 | Final, 2021 U.S. Open Cup |
| 2 | Union Omaha | 16 | 8 | 3 | 5 | 20 | 15 | +5 | 29 | 1.81 | Final |
| 3 | North Texas SC[a] | 16 | 7 | 3 | 6 | 27 | 19 | +8 | 27 | 1.69 | |
| 4 | Richmond Kickers | 16 | 8 | 6 | 2 | 22 | 22 | 0 | 26 | 1.63 | |
| 5 | Chattanooga Red Wolves SC | 15 | 6 | 5 | 4 | 21 | 17 | +4 | 22 | 1.47 | |
| 6 | FC Tucson | 16 | 6 | 6 | 4 | 21 | 19 | +2 | 22 | 1.38 | |
| 7 | Forward Madison FC | 16 | 5 | 5 | 6 | 20 | 14 | +6 | 21 | 1.31 | |
| 8 | Tormenta FC | 16 | 5 | 7 | 4 | 19 | 22 | −3 | 19 | 1.19 | |
| 9 | New England Revolution II[a] | 16 | 5 | 8 | 3 | 19 | 26 | −7 | 18 | 1.13 | |
| 10 | Fort Lauderdale CF[a] | 16 | 4 | 9 | 3 | 19 | 28 | −9 | 15 | 0.94 | |
| 11 | Orlando City B[a] | 15 | 1 | 11 | 3 | 10 | 29 | −19 | 6 | 0.40 |
Source: 2020 USL League One standings
Rules for classification: 1) points; 2) total wins; 3) total goal differential; 4) total goals scored; 5) points earned against top four Conference finishers; 6) disciplinary points; 7) coin toss or drawing of lots
Notes:
Rules for classification: 1) points; 2) total wins; 3) total goal differential; 4) total goals scored; 5) points earned against top four Conference finishers; 6) disciplinary points; 7) coin toss or drawing of lots
Notes:
- ^ a b c d Professional teams that are owned by teams playing in a higher division are ineligible for the U.S. Open Cup.