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Pitchers
- 14 Austin Amaral
- 48 Wander Arias
- 22 Jose Atencio
- 25 Bryan Caceres
- 9 Cade Cavalli #*
- 31 Brendan Collins
- 28 Riley Cornelio
- 23 Marc Davis
- 29 Mason Denaburg
- 32 Moises Diaz
- 30 Miguel Gomez
- 35 Cole Henry #*
- 19 Chance Huff
- 26 Thomas Schultz
- 40 Jared Simpson
- 35 Jarlín Susana
- 39 Mikey Tepper
- 47 Samuel Vasquez
- 15 Luke Young
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Catchers
- 43 Geraldi Diaz
- 34 Maxwell Romero Jr.
- 16 Matt Suggs
Infielders
- 27 Branden Boissiere
- 12 Marcus Brown
- 6 Armando Cruz
- 7 Gavin Dugas
- 17 Joe Naranjo
- 10 Yoander Rivero
- 13 Murphy Stehly
Outfielders
- 4 Tyler Baca #
- 3 Jared McKenzie
- 2 Roismar Quintana
- 1 Johnathon Thomas
- 20 T.J. White
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Manager
Coaches
60-day injured list
- Jake Bennett
- Peyton Glavine
- 5 Elijah Nunez
- 11 Viandel Pena
- 35 Nick Pogue
- Tyler Yankosky (full season)
 7-day injured list
* On Washington Nationals 40-man roster
~ Development list
# Rehab assignment
∞ Reserve list
‡ Restricted list
§ Suspended list
† Temporarily inactive list
Roster updated September 16, 2024
Transactions
→ More rosters: MiLB • South Atlantic League
→ Washington Nationals minor league players
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