In molecular biology, the ter site, also known as DNA replication terminus binding-site, refers to a protein domain which binds to the DNA replication terminus site. Ter-binding proteins are found in some bacterial species, and include the Tus protein which is part of the common Ter-Tus binding domain. They are required for the termination of DNA replication and function by binding to DNA replication terminator sequences, thus preventing the passage of replication forks.[1] The termination efficiency is affected by the affinity of a particular protein for the terminator sequence.
| Ter | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Nmr study of the proline repeat from tus | |||||||||
| Identifiers | |||||||||
| Symbol | Ter | ||||||||
| Pfam | PF05472 | ||||||||
| InterPro | IPR008865 | ||||||||
| SCOP2 | 5eau / SCOPe / SUPFAM | ||||||||
| |||||||||
In E. coli, there are 10 closely ter related sites encoded in the chromosome. The sites are designated TerA, TerB, ..., TerJ. Each site is 23 base pairs.[2]
Function
editA DNA replication terminus (Ter) has a role in preventing progress of the DNA replication fork.[3] Therefore, a DNA replication terminus site-binding protein binds to this site helping to block the DNA replication fork. There are two genes controlling Ter-binding activity, named tau and Tus.[1]
References
edit- ^ a b Hidaka M, Kobayashi T, Takenaka S, Takeya H, Horiuchi T (December 1989). "Purification of a DNA replication terminus (ter) site-binding protein in Escherichia coli and identification of the structural gene". J. Biol. Chem. 264 (35): 21031–7. doi:10.1016/S0021-9258(19)30040-7. PMID 2687269.
- ^ Mulcair, M.; Schaeffer, P.; Oakley, A.; Cross, H.; Neylon, C.; Hill, T.; Dixon, N. (2006). "A Molecular Mousetrap Determines Polarity of Termination of DNA Replication in E. Coli". Cell. 125 (7): 1309–1319. doi:10.1016/j.cell.2006.04.040. PMID 16814717.
- ^ Hidaka M, Kobayashi T, Horiuchi T (1991). "A newly identified DNA replication terminus site, TerE, on the Escherichia coli chromosome". J Bacteriol. 173 (1): 391–3. doi:10.1128/jb.173.1.391-393.1991. PMC 207198. PMID 1824765.