Thermococcus chitonophagus is a chitin-degrading, hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent. T. chitonophagus was discovered off the West of the Mexican Coast 2,600m in the water. Since there was DNA–DNA hybridization, 16S rRNA, and the GC-content was 46.5 mol%, upon analysis, the chitonophagus were identified to be of the Thermococcus genus. It is anaerobic, round to slightly irregular coccus-shaped, 1.2–2.5 μm in diameter, and motile by means of a tuft of flagella.[1]
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| Species: | T. chitonophagus
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| Thermococcus chitonophagus Huber and Stetter 1996
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T. chitonophagus is one of only three species of archaeon that can grow on chitin. The chitinase was isolated and found to have a molecular weight of 70 kDa. It retains 50% of its activity after an hour at 120 °C.[2]
References
edit- ^ Huber, R.; Stöhr, Josef; Hohenhaus, Sabine; Rachel, Reinhard; Burggraf, Siegfried; Jannasch, Holger W.; Stetter, Karl O. (1995). "Thermococcus chitonophagus sp. nov., a novel, chitin-degrading, hyperthermophilic archaeum from a deep-sea hydrothermal vent environment". Archives of Microbiology. 164 (4): 255–264. doi:10.1007/s002030050262. ISSN 0302-8933. S2CID 36502509.
- ^ Yves Le Gal; Roland Ulber; Garabed Antranikian (2005-09-29). Marine Biotechnology I. Vol. 1. Springer Science and Business Media. p. 288. ISBN 3540256598. Retrieved 2016-09-28.
Further reading
edit- Andronopoulou E; Vorgias CE (2003). "Purification and characterization of a new hyperthermostable, allosamidin-insensitive and denaturation-resistant chitinase from the hyperthermophilic archaeon Thermococcus chitonophagus". Extremophiles. 7 (1): 43–53. doi:10.1007/s00792-002-0294-3. PMID 12579379. S2CID 21502608.
- Andronopoulou E; Vorgias CE (2004). "Isolation, cloning, and overexpression of a chitinase gene fragment from the hyperthermophilic archaeon Thermococcus chitonophagus: semi-denaturing purification of the recombinant peptide and investigation of its relation with other chitinases". Protein Expr Purif. 35 (2): 264–71. doi:10.1016/j.pep.2004.02.002. PMID 15135401.
- Andronopoulou, E; Vorgias, CE (2004). "Multiple components and induction mechanism of the chitinolytic system of the hyperthermophilic archaeon Thermococcus chitonophagus". Applied Microbiology and Biotechnology. 65 (6): 694–702. doi:10.1007/s00253-004-1640-4. PMID 15322771. S2CID 22813106.
External links
edit- "Thermococcus chitonophagus" at the Encyclopedia of Life
- LPSN
- WORMS entry
- Type strain of Thermococcus chitonophagus at BacDive - the Bacterial Diversity Metadatabase
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