Twelve Mile or Twelvemile may refer to:
- Twelve Mile, Indiana, an unincorporated community
- Twelvemile, Missouri, an unincorporated community
- Twelve Mile Circle, part of the Pennsylvania–Delaware boundary
- Twelve Mile Township in Williams County, North Dakota, USA
- Twelve Mile Lake Township in Emmet County, Iowa, USA
- Twelvemile Township, Madison County, Missouri
- Twelve Mile, New South Wales, Australia
- Twelve Mile Creek, New South Wales, Australia
- A twelve nautical mile territorial waters limit