UDP-N-acetylglucosamine 2-epimerase (hydrolysing) (EC 3.2.1.183, UDP-N-acetylglucosamine 2-epimerase, GNE (gene), siaA (gene), neuC (gene)) is an enzyme with systematic name UDP-N-acetyl-alpha-D-glucosamine hydrolase (2-epimerising).[1][2][3][4] This enzyme catalyses the following chemical reaction
| UDP-N-acetylglucosamine 2-epimerase (hydrolysing) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.2.1.183 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- UDP-N-acetyl-alpha-D-glucosamine + H2O N-acetyl-D-mannosamine + UDP
The enzyme is found in mammalian liver, as well as in some pathogenic bacteria including Neisseria meningitidis and Staphylococcus aureus.
References
edit- ^ Stäsche R, Hinderlich S, Weise C, Effertz K, Lucka L, Moormann P, Reutter W (September 1997). "A bifunctional enzyme catalyzes the first two steps in N-acetylneuraminic acid biosynthesis of rat liver. Molecular cloning and functional expression of UDP-N-acetyl-glucosamine 2-epimerase/N-acetylmannosamine kinase". The Journal of Biological Chemistry. 272 (39): 24319–24. doi:10.1074/jbc.272.39.24319. PMID 9305888.
- ^ Chou WK, Hinderlich S, Reutter W, Tanner ME (March 2003). "Sialic acid biosynthesis: stereochemistry and mechanism of the reaction catalyzed by the mammalian UDP-N-acetylglucosamine 2-epimerase". Journal of the American Chemical Society. 125 (9): 2455–61. doi:10.1021/ja021309g. PMID 12603133.
- ^ Blume A, Ghaderi D, Liebich V, Hinderlich S, Donner P, Reutter W, Lucka L (June 2004). "UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, functionally expressed in and purified from Escherichia coli, yeast, and insect cells". Protein Expression and Purification. 35 (2): 387–96. doi:10.1016/j.pep.2004.02.013. PMID 15135418.
- ^ Murkin AS, Chou WK, Wakarchuk WW, Tanner ME (November 2004). "Identification and mechanism of a bacterial hydrolyzing UDP-N-acetylglucosamine 2-epimerase". Biochemistry. 43 (44): 14290–8. doi:10.1021/bi048606d. PMID 15518580.
External links
edit- UDP-N-acetylglucosamine+2-epimerase+(hydrolysing) at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
