USS Nightingale may refer to the following ships of the United States Navy:
- USS Nightingale (1851), was a slave ship captured in 1861 and sold in 1865
- USS Nightingale (SP-523), was a motorboat acquired by the US Navy in 1917 and sold in 1919
- USS Nightingale (AMc-18), was a coastal minesweeper acquired by the US Navy in 1940 and returned to the owner in 1944
- USS Nightingale (AMc-149), was acquired by the US Navy in 1941 and struck from the Navy List in 1946
- USS Nightingale (YMS-290), was a minesweeper commissioned in 1943 and struck from the Navy List in 1959