Uncle John may refer to:
- Uncle John (film), a 2015 American thriller film
- Uncle John's (store), a Philippine convenience store chain
- Uncle John's Bathroom Reader, a series of books containing trivia and short essays on miscellaneous topics
- John McCarthy (computer scientist) (1927–2011), computer scientist known as "Uncle John"
- Uncle John an Indian ice cream brand founded by George Verghese
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