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Transmembrane protein 151B

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Transmembrane protein 151B is the protein encoded by the TMEM151B gene.[1]

Nomenclature

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The Transmembrane protein 151B gene is also known as TMEM151B, C6orf137, and bA444E17.5.[1]

Gene

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The gene is located on the positive strand of chromosome 6 at the location 6p21.1 from the chromosome position 44270450 to 44279444, for a total length of 8,995 base pairs.[2] It is a complex locus that contains both TMEM151B and SPATS1. [3] TMEM151B has one paralog: TMEM151A.[4]

Transcript

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The mRNA contains 3 exons, with a transcribed mRNA length of 4911bp, and the coding region containing 1701bp. [2]

 
Visualization of the TMEM151B gene. The green lines above the diagram show other promotors within the gene.

Protein

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2D visualization of the TMEM151B protein using Protter.[5]
 
3D model of the TMEM151B protein using Alpha Fold.

The protein has a length of 566 amino acids and contains two transmembrane domains.[1] According to Compute pI/Mw,  molecular weight of approximately 61 kDa, which matches the weight listed on NCBI, and a theoretical isoelectric point of 6.72[6][1]. The human TMEM151B protein composition is poor in lysine and arginine, lysine comprising 1.4% of the amino acids and arginine making up 0.8% of the total protein. The mouse ortholog is also arginine poor. [7]

Expression

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RNA expression map of TMEM151B within the mouse brain[8]

RNA-seq gene expression profiling shows high expression in the brain, and notable expression in the testes.[9] NCBI geo profiles similarly show localization to the brain tissues.[10] Within the mouse brain, TMEM151B has high expression particularly within the cerebellum, medulla, and olfactory bulb according to the Allen Brain Atlas. [8]

Protein interactions

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The TMEM151B protein interacts with one other protein according to BioGRID.[11]

  • SREBF2- a transcription factor precursor which is imbedded in the endoplasmic reticulum membrane; activates genes involved with cholesterol biosynthesis.[12]

Homology and Evolution

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Evolutionary rate of TMEM151B compared to that of Fibrinogen Alpha Chain and Cytochrome C.

The TMEM151B gene is conserved within most vertebrates, and appears to be conserved within some invertebrates. Its paralog, TMEM151A, has a 47.7% sequence identity with TMEM151B.[4][13] The evolution rate is relatively slow: between the rate of change in fibrinogen alpha and cytochrome c.[4][14]

 
Ortholog space for TMEM151B
 
Table of orthologs for the TMEM151B gene, found using NCBI Blast[4]

Research

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Alternative splicing of TMEM151B along with 4 other genes were linked to colorectal cancer[15]. It was also found to be up-regulated in post-menopausal breast cancer[16][17]. A SNP found within TMEM151B is associated with the development of lean muscle.[18]


References

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  1. ^ a b c d "transmembrane protein 151B [Homo sapiens] - Protein - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2021-09-29.
  2. ^ a b "Homo sapiens transmembrane protein 151B (TMEM151B), mRNA - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2021-09-29.{{cite web}}: CS1 maint: url-status (link)
  3. ^ "AceView: Gene:TMEM151BandSPATS1, a comprehensive annotation of human, mouse and worm genes with mRNAs or ESTsAceView". www.ncbi.nlm.nih.gov. Retrieved 2021-10-04.
  4. ^ a b c d "BLAST: Basic Local Alignment Search Tool". blast.ncbi.nlm.nih.gov. Retrieved 2022-01-13.
  5. ^ "Protter - interactive protein feature visualization". wlab.ethz.ch. Retrieved 2022-01-13.
  6. ^ "ExPASy - Compute pI/Mw tool". web.expasy.org. Retrieved 2022-01-13.
  7. ^ "SAPS < Sequence Statistics < EMBL-EBI". www.ebi.ac.uk. Retrieved 2022-01-13.
  8. ^ a b "ISH Data :: Allen Brain Atlas: Mouse Brain". mouse.brain-map.org. Retrieved 2022-01-13.
  9. ^ "TMEM151B transmembrane protein 151B [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2022-01-13.
  10. ^ "GDS596 / 213678_at". www.ncbi.nlm.nih.gov. Retrieved 2022-01-13.
  11. ^ "TMEM151B (RP11-444E17.5) Result Summary | BioGRID". thebiogrid.org. Retrieved 2022-01-13.
  12. ^ "SREBF2 Gene - GeneCards | SRBP2 Protein | SRBP2 Antibody". www.genecards.org. Retrieved 2022-01-13.
  13. ^ "Clustal Omega < Multiple Sequence Alignment < EMBL-EBI". www.ebi.ac.uk. Retrieved 2022-01-13.
  14. ^ "TimeTree :: The Timescale of Life". www.timetree.org. Retrieved 2022-01-13.
  15. ^ Liu, Jingwei; Li, Hao; Shen, Shixuan; Sun, Liping; Yuan, Yuan; Xing, Chengzhong (2018). "Alternative splicing events implicated in carcinogenesis and prognosis of colorectal cancer". Journal of Cancer. 9 (10): 1754–1764. doi:10.7150/jca.24569. ISSN 1837-9664.
  16. ^ Carter, JM; Nair, AA; Davila, JI; Heinzen, EP; Hoskin, TL; Winham, SJ; Radisky, DC; Visscher, DW; Degnim, AC (2019-02-15). "Abstract P3-08-10: A unique coding and non-coding benign breast transcriptome in post-menopausal ER+ breast cancer". Poster Session Abstracts. American Association for Cancer Research. doi:10.1158/1538-7445.sabcs18-p3-08-10.
  17. ^ Qian, Da; Zheng, Qinghui; Wu, Danping; Ye, Buyun; Qian, Yangyang; Zhou, Tao; Qiu, Jie; Meng, Xuli (2021-05-13). "Integrated Analysis of ceRNA Network Reveals Prognostic and Metastasis Associated Biomarkers in Breast Cancer". Frontiers in Oncology. 11. doi:10.3389/fonc.2021.670138. ISSN 2234-943X.{{cite journal}}: CS1 maint: unflagged free DOI (link)
  18. ^ Pei, Yu-Fang; Hu, Wen-Zhu; Yang, Xiao-Lin; Wei, Xin-Tong; Feng, Gui-Juan; Zhang, Hong; Shen, Hui; Tian, Qing; Deng, Hong-Wen; Zhang, Lei (2019-11-23). "Two functional variants at 6p21.1 were associated with lean mass". Skeletal Muscle. 9 (1). doi:10.1186/s13395-019-0212-3. ISSN 2044-5040.{{cite journal}}: CS1 maint: unflagged free DOI (link)
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