Wikipedia

Kedrosolan

Joined 25 January 2018

Potential topics I would like to edit for our seminar:

  1. Gluconeogenesis
  2. Coenzyme A
  3. Flavin adenine dinucleotide

All of the above have a lot of information and probably not enough work for expansion/improvement

  1. Phosphofructokinase 2

There is a lot there, but still needs more work.

  1. Phosphoenolpyruvic acid: even though it short there isn't a lot more to stay about it.

Week 4 Research Results for chosen topic: Phosphofructo kinase 2

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The main objectives I have in contributing to this article include both expanding upon content and improving the visual aids on the site. I would like to clarify more about the reaction mechanism, more specifically emphasize the bi-functionality of this enzyme, with both editing the verbal descriptions but also adding images of both states of the enzyme (PF2/FBPase2).

For photos and images, I intend to generate images myself via programs - I am planning to ask Professor Tienson about recommended programs for creating more visually insightful protein models. (Is PDB sufficient?) I liked the protein images on the page of closely related Adenylate kinase (particularly the 3D color-coded ribbon/surface model along with the close-up image of the active site, and hope to incorporate something similar for this page.

Here are several articles I looked up to gain more insight into how Phosphofructo kinase 2 works and which will guide me in editing this article. I obtained all of them via Pubmed.

  1. I liked a lot of the photos in this article and how it already had created image of transition state for PFK-2 phosphotransferase catalysis.[1]
  2. New primary research which succinctly explains PFK-2 function in background section.[2] --> Linked to this, which is a review article which gave me a better understanding of why PFK2's control of fructose 2,6-biphosphate is important.[3]
  3. Still deciding how to concisely explain role it plays in liver
    1. Best review summary of overall hepatic metabolism[4]
    2. Discussion of glucagon interruption in PFK2 binding in hepatic glucose metabolism[5]
  4. Pancreas cell discussion[6]
  5. Another review article of hepatic glucokinase metabolism -- Make sure to view using UCLA wifi* -- accessed via UCLA wifi but unable to create citation without! (http://www.annualreviews.org/doi/10.1146/annurev-nutr-071715-051145)
  6. Textbook explanation! VERY helpful about mechanism plus images[7]

To do: Work on utilizing more secondary, tertiary sources?

References

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  1. ^ Rider, Mark H.; Bertrand, Luc; Vertommen, Didier; Michels, Paul A.; Rousseau, Guy G.; Hue, Louis (2004-08-01). "6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase: head-to-head with a bifunctional enzyme that controls glycolysis". Biochemical Journal. 381 (3): 561–579. doi:10.1042/bj20040752. ISSN 0264-6021. PMID 15170386.
  2. ^ Marsin, A-S.; Bertrand†, L.; Rider, M.H.; Deprez, J.; Beauloye, C.; Vincent‡, M.F.; Berghe‡, G. Van den; Carling, D.; Hue, L. "Phosphorylation and activation of heart PFK-2 by AMPK has a role in the stimulation of glycolysis during ischaemia". Current Biology. 10 (20): 1247–1255. doi:10.1016/s0960-9822(00)00742-9.
  3. ^ Hue, L.; Rider, M. H. (1987-07-15). "Role of fructose 2,6-bisphosphate in the control of glycolysis in mammalian tissues". Biochemical Journal. 245 (2): 313–324. doi:10.1042/bj2450313. ISSN 0264-6021. PMID 2822019.
  4. ^ Payne, Victoria A.; Arden, Catherine; Wu, Chaodong; Lange, Alex J.; Agius, Loranne (July 2005). "Dual role of phosphofructokinase-2/fructose bisphosphatase-2 in regulating the compartmentation and expression of glucokinase in hepatocytes". Diabetes. 54 (7): 1949–1957. ISSN 0012-1797. PMID 15983194.
  5. ^ Cullen, Kirsty S.; Al-Oanzi, Ziad H.; O'Harte, Finbarr P.M.; Agius, Loranne; Arden, Catherine. "Glucagon induces translocation of glucokinase from the cytoplasm to the nucleus of hepatocytes by transfer between 6-phosphofructo 2-kinase/fructose 2,6-bisphosphatase-2 and the glucokinase regulatory protein". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1843 (6): 1123–1134. doi:10.1016/j.bbamcr.2014.02.006.
  6. ^ Arden, Catherine; Hampson, Laura J.; Huang, Guo C.; Shaw, James A. M.; Aldibbiat, Ali; Holliman, Graham; Manas, Derek; Khan, Salmaan; Lange, Alex J. (2008-04-01). "A role for PFK-2/FBPase-2, as distinct from fructose 2,6-bisphosphate, in regulation of insulin secretion in pancreatic β-cells". Biochemical Journal. 411 (1): 41–51. doi:10.1042/bj20070962. ISSN 0264-6021. PMID 18039179.
  7. ^ Berg, Jeremy M.; Tymoczko, John L.; Stryer, Lubert (2002). "The Glycolytic Pathway Is Tightly Controlled". {{cite journal}}: Cite journal requires |journal= (help)
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