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Transposon Site Hybridization (TraSH) Transposon Site Hybridization (TraSH) is a molecular genetics method developed by Christopher Sassetti and Eric Rubin at Harvard University School of Public Heath in 2001[1]. It allows researchers to screen a library of bacterial mutants for their relative fitness under various conditions. The original TraSH library was constructed in Mycobacterium bovis BCG, and an additional library has been made for M. tuberculosis.
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