Mechanism
editEndogenous trans-acting siRNAs (ta-siRNAs) act via hetero-silencing, which means that the genes they target for cleavage and repression do not have much resemblance to the genes from which the siRNAs derive. This differs from other endogenous siRNAs which are cis-acting and perform auto-silencing, repressing the expression of genes that are the same as or have a lot of resemblance to the genes from which they derive.[1] A member of the Argonaute protein family is a component of all RNA silencing effector complexes. [2] AGO7/ZIPPY appears to play a role in the ta-siRNA pathway in Arabidopsis by acting during TAS3-derived ta-siRNA-mediated regulation. [3]
References:
- ^ Vazquez, Franck; Vaucheret, Hervé; Rajagopalan, Ramya; Lepers, Christelle; Gasciolli, Virginie; Mallory, Allison C.; Hilbert, Jean-Louis; Bartel, David P.; Crété, Patrice (1 October 2004). "Endogenous trans-Acting siRNAs Regulate the Accumulation of Arabidopsis mRNAs". Molecular Cell. 16 (1): 69–79. doi:10.1016/j.molcel.2004.09.028. PMID 15469823.
{{cite journal}}: CS1 maint: date and year (link) - ^ Tomari, Yukihide; Zamore, Phillip D. (1 March 2005). "Perspective: machines for RNAi". Genes & Development. 19 (5): 517–529. doi:10.1101/gad.1284105. PMID 15741316.
- ^ Adenot, Xavier; Elmayan, Taline; Lauressergues, Dominique; Boutet, Stéphanie; Bouché, Nicolas; Gasciolli, Virginie; Vaucheret, Hervé (1 May 2006). "DRB4-Dependent TAS3 trans-Acting siRNAs Control Leaf Morphology through AGO7". Current Biology. 16 (9): 927–932. doi:10.1016/j.cub.2006.03.035. PMID 16682354.