User:Lulabees/Phospholipase C

Article Draft

Lead

Phospholipase C (PLC) is a class of membrane-associated enzymes that cleave phospholipids just before the phosphate group (see figure). It is most commonly taken to be synonymous with the human forms of this enzyme, which play an important role in eukaryotic cell physiology, in particular signal transduction pathways. Phospholipase C's role in signal transduction is its cleavage of phosphatidylinositol 4,5-bisphosphate (PIP₂) into diacyl glycerol (DAG) and inositol 1,4,5-trisphosphate (IP₃), which serve as second messengers. Activators of each PLC vary, but typically include heterotrimeric G protein subunits, protein tyrosine kinases, small G proteins, Ca²⁺, and phospholipids.

There are thirteen kinds of mammalian phospholipase C that are classified into six isotypes (β, γ, δ, ε, ζ, η) according to structure. Each PLC has unique and overlapping controls over expression and subcellular distribution.

Variants

Mammalian

The extensive number of functions exerted by the PLC reaction requires that it be strictly regulated and able to respond to multiple extra- and intracellular inputs with appropriate kinetics. This need has guided the evolution of six isotypes of PLC in animals, each with a distinct mode of regulation. The pre-mRNA of PLC can also be subject to differential splicing such that a mammal may have up to 30 PLC enzymes.

• beta: PLCB1, PLCB2, PLCB3, PLCB4
• gamma: PLCG1, PLCG2
• delta: PLCD1, PLCD3, PLCD4
• epsilon: PLCE1
• eta: PLCH1, PLCH2
• zeta: PLCZ1
• phospholipase C-like: PLCL1, PLCL2

Bacterial

Most of the bacterial variants of phospholipase C are characterized into one of four groups of structurally related proteins. The toxic phospholipases C are capable of interacting with eukaryotic cell membranes and hydrolyzing phosphatidylcholine and sphingomyelin, ultimately leading to cell lysis.

• Zinc-metallophospholipases C: Clostridium perfringens alpha-toxin, Bacillus cereus PLC (BC-PLC)
• Sphingomyelinases: B. cereus, Staphylococcus aureus
• Phosphatidylinositol-hydrolyzing enzymes: B. cereus, B. thuringiensis, L. monocytogenes (PLC-A)
• Pseudomonad phospholipases C: Pseudomonas aeruginosa (PLC-H and PLC-N)

Chromadorea

The class of Chromadorea also utilizes the enzyme phospholipase C to regulate the releases of calcium. The enzyme releases inositol 1,4,5-trisphosphate (IP3) that denotes a signaling pathway involved in activating ovulation, the propelling of the oocyte into the spermatheca. The expression of the enzyme in the spermatheca is controlled by the transcription factors FOS-1 and JUN-1. This gene is involved in various activities like controlling GTPase, breaking down certain molecules, and binding to small GTPase. It helps in fighting bacteria and regulating protein movement in cells. It's found in different parts of the body like the excretory system, intestines, nerves, and reproductive organs.

Enzyme Structure

In mammals, PLCs share a conserved core structure and differ in other domains specific to each family. The core enzyme includes a split triosephosphate isomerase (TIM) barrel, Pleckstrin homology (PH) domain, four tandem EF hand domains, and a C2 domain. The TIM barrel contains the active site, all catalytic residues, and a Ca²⁺ binding site. It has an autoinhibitory insert that interrupts its activity called an X-Y linker. The X-Y linker has been shown to occlude the active site, and with its removal, PLC is activated.

The genes encoding alpha-toxin (Clostridium perfringens), Bacillus cereus PLC (BC-PLC), and PLCs from Clostridium bifermentans and Listeria monocytogenes have been isolated and nucleotides sequenced. The sequences have significant homology, approximately 250 residues, from the N-terminus. Alpha-toxin has an additional 120 residues in the C-terminus. The C-terminus of the alpha-toxin has been reported as a "C2-like" domain, referencing the C2 domain found in eukaryotes that are involved in signal transduction and present in mammalian phosphoinositide phospholipase C.

Enzyme Mechanism

The primary catalyzed reaction of PLC occurs on an insoluble substrate at a lipid-water interface. The residues in the active site are conserved in all PLC isotypes. In animals, PLC selectively catalyzes the hydrolysis of the phospholipid phosphatidylinositol 4,5-bisphosphate (PIP₂) on the glycerol side of the phosphodiester bond. There is the formation of a weakly enzyme-bound intermediate, inositol 1,2-cyclic phosphodiester, and release of diacylglycerol (DAG). The intermediate is then hydrolyzed to inositol 1,4,5-trisphosphate (IP₃). Thus the two end products are DAG and IP₃. The acid/base catalysis requires two conserved histidine residues and a Ca²⁺ ion is needed for PIP₂ hydrolysis. It has been observed that the active-site Ca²⁺ coordinates with four acidic residues and if any of the residues are mutated then a greater Ca²⁺ concentration is needed for catalysis.

Signaling Pathway

File:PLC Signaling.jpeg

Phospholipase C Signaling Pathway

Phosphoinositide-specific phospholipase C (PLC) is a key player in cell signaling processes. When cells encounter signals like hormones or growth factors, PLC breaks down a molecule called PIP2 to produce new signaling molecules. Various types of PLC are activated differently, contributing to cells' ability to respond to their surroundings.

Activation

Receptors that activate this pathway are mainly G protein-coupled receptors coupled to the G_αq subunit, including:

• 5-HT₂ serotonergic receptors
• α₁ (Alpha-1) adrenergic receptors
• Calcitonin receptors
• H₁ histamine receptors
• Metabotropic glutamate receptors, Group I
• M₁, M₃, and M₅ muscarinic receptors
• Thyroid-Releasing Hormone receptor in anterior pituitary gland

Other, minor, activators than G_αq are:

• MAP kinase. Activators of this pathway include PDGF and FGF.
• βγ-complex of heterotrimeric G-proteins, as in a minor pathway of growth hormone release by growth hormone-releasing hormone.
• Cannabinoid receptors

Inhibition

• Small molecule U73122: aminosteroid, putative PLC inhibitor. However, the specificity of U73122 has been questioned. It has been reported that U73122 activates the phospholipase activity of purified PLCs.
• Edelfosine: lipid-like, anti-neoplastic agent (ET-18-OCH3)
• Autoinhibition of X-Y linker in mammalian cells: It is proposed that the X-Y linker consists of long stretches of acidic amino acids that form dense areas of negative charge. These areas could be repelled by the negatively charged membrane upon binding of the PLC to membrane lipids. The combination of repulsion and steric constraints is thought to remove the X-Y linker from near the active site and relieve auto-inhibition.
• Compounds containing the morpholinobenzoic acid scaffold belong to a class of drug-like phosphatidylcholine-specific PLC inhibitors.
• o-phenanthroline: heterocyclic organic compound, known to inhibit zinc-metalloenzymes
• EDTA: molecule that chelates Zn²⁺ ions and effectively inactivates PLC, known to inhibit zinc-metalloenzymes

Biological Function

The two products of the PLC catalyzed reaction, DAG and IP₃, are important second messengers that control diverse cellular processes and are substrates for synthesis of other important signaling molecules. When PIP₂ is cleaved, DAG remains bound to the membrane, and IP₃ is released as a soluble structure into the cytosol. IP₃ then diffuses through the cytosol to bind to IP₃ receptors, particularly calcium channels in the smooth endoplasmic reticulum (ER). This causes the cytosolic concentration of calcium to increase, causing a cascade of intracellular changes and activity. In addition, calcium and DAG together work to activate protein kinase C, which goes on to phosphorylate other molecules, leading to altered cellular activity. End-effects include taste, tumor promotion, as well as vesicle exocytosis, superoxide production from NADPH oxidase, and JNK activation.

Both DAG and IP₃ are substrates for the synthesis of regulatory molecules. DAG is the substrate for the synthesis of phosphatidic acid, a regulatory molecule. IP₃ is the rate-limiting substrate for the synthesis of inositol polyphosphates, which stimulate multiple protein kinases, transcription, and mRNA processing. Regulation of PLC activity is thus vital to the coordination and regulation of other enzymes of pathways that are central to the control of cellular physiology.

Further information: Calcium function in vertebrates and Function of protein kinase C

Additionally, phospholipase C plays an important role in the inflammation pathway. The binding of agonists such as thrombin, epinephrine, or collagen, to platelet surface receptors can trigger the activation of phospholipase C to catalyze the release of arachidonic acid from two major membrane phospholipids, phosphatidylinositol and phosphatidylcholine. Arachidonic acid can then go on into the cyclooxygenase pathway (producing prostaglandins (PGE1, PGE2, PGF2), prostacyclins (PGI2), or thromboxanes (TXA2)), and the lipoxygenase pathway (producing leukotrienes (LTB4, LTC4, LTD4, LTE4)).

The bacterial variant Clostridium perfringens type A produces alpha-toxin. The toxin has phospholipase C activity, and causes hemolysis, lethality, and dermonecrosis. At high concentrations, alpha-toxin induces massive degradation of phosphatidylcholine and sphingomyelin, producing diacylglycerol and ceramide, respectively. These molecules then participate in signal transduction pathways. It has been reported that the toxin activates the arachidonic acid cascade in isolated rat aorta. The toxin-induced contraction was related to generation of thromboxane A₂ from arachidonic acid. Thus it is likely the bacterial PLC mimics the actions of endogenous PLC in eukaryotic cell membranes.

References

Kadamur G, Ross EM (2013). "Mammalian phospholipase C". Annual Review of Physiology. 75: 127–54. doi:10.1146/annurev-physiol-030212-183750. PMID 23140367.

Suh, PG; Park, JI; Manzoli, L; Cocco, L; Peak, JC; Katan, M; Fukami, K; Kataoka, T; Yun, S; Ryu, SH (2008). "Multiple roles of phosphoinositide-specific phospholipase C isozymes". BMB Reports. 41 (6): 415–34. doi:10.5483/bmbrep.2008.41.6.415. hdl:11585/62661. PMID 18593525.

Titball, RW (1993). "Bacterial phospholipases C." Microbiological Reviews. 57 (2): 347–66. doi:10.1128/MMBR.57.2.347-366.1993. PMC 372913. PMID 8336671.

Hicks SN, Jezyk MR, Gershburg S, Seifert JP, Harden TK, Sondek J (August 2008). "General and versatile autoinhibition of PLC isozymes". Molecular Cell. 31 (3): 383–94. doi:10.1016/j.molcel.2008.06.018. PMC 2702322. PMID 18691970.

Sakurai J, Nagahama M, Oda M (November 2004). "Clostridium perfringens alpha-toxin: characterization and mode of action". Journal of Biochemistry. 136 (5): 569–74. doi:10.1093/jb/mvh161. PMID 15632295.

Essen LO, Perisic O, Katan M, Wu Y, Roberts MF, Williams RL (February 1997). "Structural mapping of the catalytic mechanism for a mammalian phosphoinositide-specific phospholipase C". Biochemistry. 36 (7): 1704–18. doi:10.1021/bi962512p. PMID 9048554.

Ellis, MV; James, SR; Perisic, O; Downes, PC; Williams, RL; Katan, M (1998). "Catalytic Domain of Phosphoinositide-specific Phospholipase C (PLC): mutation analysis of residues within the active site of hydrophobic ridge of PLCD1". The Journal of Biological Chemistry. 273 (19): 11650–9. doi:10.1074/jbc.273.19.11650. PMID 9565585.

Walter F. Boron (2003). Medical Physiology: A Cellular And Molecular Approaoch. Elsevier/Saunders. p. 1300. ISBN 978-1-4160-2328-9. Page 104

GeneGlobe -> GHRH Signaling[permanent dead link] Retrieved on May 31, 2009

Bleasdale JE, Thakur NR, Gremban RS, Bundy GL, Fitzpatrick FA, Smith RJ, Bunting S (November 1990). "Selective inhibition of receptor-coupled phospholipase C-dependent processes in human platelets and polymorphonuclear neutrophils". The Journal of Pharmacology and Experimental Therapeutics. 255 (2): 756–68. PMID 2147038.

Macmillan D, McCarron JG (July 2010). "The phospholipase C inhibitor U-73122 inhibits Ca(2+) release from the intracellular sarcoplasmic reticulum Ca(2+) store by inhibiting Ca(2+) pumps in smooth muscle". British Journal of Pharmacology. 160 (6): 1295–301. doi:10.1111/j.1476-5381.2010.00771.x. PMC 2938802. PMID 20590621.

Huang W, Barrett M, Hajicek N, Hicks S, Harden TK, Sondek J, Zhang Q (February 2013). "Small molecule inhibitors of phospholipase C from a novel high-throughput screen". The Journal of Biological Chemistry. 288 (8): 5840–8. doi:10.1074/jbc.M112.422501. PMC 3581404. PMID 23297405.

Klein RR, Bourdon DM, Costales CL, Wagner CD, White WL, Williams JD, Hicks SN, Sondek J, Thakker DR (April 2011). "Direct activation of human phospholipase C by its well known inhibitor u73122". The Journal of Biological Chemistry. 286 (14): 12407–16. doi:10.1074/jbc.M110.191783. PMC 3069444. PMID 21266572.

Horowitz LF, Hirdes W, Suh BC, Hilgemann DW, Mackie K, Hille B (September 2005). "Phospholipase C in living cells: activation, inhibition, Ca2+ requirement, and regulation of M current". The Journal of General Physiology. 126 (3): 243–62. doi:10.1085/jgp.200509309. PMC 2266577. PMID 16129772.

Rees, Shaun W. P.; Leung, Euphemia; Reynisson, Jóhannes; Barker, David; Pilkington, Lisa I. (2021-09-01). "Development of 2-Morpholino-N-hydroxybenzamides as anti-proliferative PC-PLC inhibitors". Bioorganic Chemistry. 114: 105152. doi:10.1016/j.bioorg.2021.105152. ISSN 0045-2068. PMID 34328856.

Eurtivong, C.; Pilkington, L. I.; van Rensburg, M.; White, R. M.; Kaur Brar, H.; Rees, S.; Paulin, E. K.; Xu, C. S.; Sharma, N.; Leung, I. K. H.; Leung, E.; Barker, D.; Reynisson, J. (1 February 2020). "Discovery of novel phosphatidylcholine-specific phospholipase C drug-like inhibitors as potential anticancer agents". European Journal of Medicinal Chemistry. 187: 111919. doi:10.1016/j.ejmech.2019.111919. PMID 31810783. S2CID 208813280.

Pilkington, L. I.; Sparrow, K.; Rees, S. W. P.; Paulin, E. K.; van Rensburg, M.; Xu, C. S.; Langley, R. J.; Leung, I. K. H.; Reynisson, J.; Leung, E.; Barker, D. (2020). "Development, Synthesis and Biological Investigation of a Novel Class of Potent PC-PLC Inhibitors". European Journal of Medicinal Chemistry. 191: 112162. doi:10.1016/j.ejmech.2020.112162. PMID 32101781. S2CID 211536972.

Little C, Otnåss AB (June 1975). "The metal ion dependence of phospholipase C from Bacillus cereus". Biochimica et Biophysica Acta (BBA) - Enzymology. 391 (2): 326–33. doi:10.1016/0005-2744(75)90256-9. PMID 807246.

"Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus" (PDF). Product Information. Sigma Aldrich.

Hilgemann DW (October 2007). "Local PIP(2) signals: when, where, and how?". Pflügers Archiv. 455 (1): 55–67. doi:10.1007/s00424-007-0280-9. PMID 17534652. S2CID 29839094.

Hansen (1 May 2015). "Lipid agonism: The PIP2 paradigm of ligand-gated ion channels". Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1851 (5): 620–628. doi:10.1016/j.bbalip.2015.01.011. PMC 4540326. PMID 25633344.

Falkenburger BH, Jensen JB, Dickson EJ, Suh BC, Hille B (September 2010). "Phosphoinositides: lipid regulators of membrane proteins". The Journal of Physiology. 588 (Pt 17): 3179–85. doi:10.1113/jphysiol.2010.192153. PMC 2976013. PMID 20519312.

Alberts B, Lewis J, Raff M, Roberts K, Walter P (2002). Molecular biology of the cell (4th ed.). New York: Garland Science. ISBN 978-0-8153-3218-3.

Li Z, Jiang H, Xie W, Zhang Z, Smrcka AV, Wu D (February 2000). "Roles of PLC-beta2 and -beta3 and PI3Kgamma in chemoattractant-mediated signal transduction". Science. 287 (5455): 1046–9. Bibcode:2000Sci...287.1046L. doi:10.1126/science.287.5455.1046. PMID 10669417.

Gresset A, Sondek J, Harden TK (2012). "The phospholipase C isozymes and their regulation". Phosphoinositides I: Enzymes of Synthesis and Degradation. Subcellular Biochemistry. Vol. 58. pp. 61–94. doi:10.1007/978-94-007-3012-0_3. ISBN 978-94-007-3011-3. PMC 3638883. PMID 22403074.

Piomelli, Daniele (1993-04-01). "Arachidonic acid in cell signaling" (PDF). Current Opinion in Cell Biology. 5 (2): 274–280. doi:10.1016/0955-0674(93)90116-8. PMID 7685181.

Fujii Y, Sakurai J (May 1989). "Contraction of the rat isolated aorta caused by Clostridium perfringens alpha toxin (phospholipase C): evidence for the involvement of arachidonic acid metabolism". British Journal of Pharmacology. 97 (1): 119–24. doi:10.1111/j.1476-5381.1989.tb11931.x. PMC 1854495. PMID 2497921.

Singaravelu, Gunasekaran; Singson, Andrew (2013-1). "Calcium signaling surrounding fertilization in the nematode Caenorhabditis elegans". Cell calcium. 53 (1): 2–9. doi:10.1016/j.ceca.2012.11.009. ISSN 0143-4160. PMC 3566351. PMID 23218668.